Owen D A, Pipkin M A
J Pharmacol Methods. 1985 Jul;13(4):309-15. doi: 10.1016/0160-5402(85)90012-9.
A technique is described that allows simultaneous and quantitative assay of the action of agonists and antagonists at histamine H1- and H2-receptors in anesthetized guinea pigs. The principle of the technique is based on H1-receptor bronchoconstriction and H2-receptor tachycardia. Studies have been made to assay antagonists at either receptor and provide simultaneous evidence of selectivity or to assay combined antagonists such as Smith Kline & French 93319. Studies with histamine receptor agonists provide evidence of the potency and selectivity of these compounds relative to histamine.
本文描述了一种技术，可同时定量测定麻醉豚鼠体内组胺H1和H2受体上激动剂和拮抗剂的作用。该技术原理基于H1受体介导的支气管收缩和H2受体介导的心动过速。已开展研究来测定任一受体上的拮抗剂，并同时提供其选择性证据，或测定联合拮抗剂，如史克必成93319。对组胺受体激动剂的研究提供了这些化合物相对于组胺的效价和选择性证据。
