Wang Futing, Deng Suping, Song Changxiao, Fu Xiaofei, Zhang Ningbo, Li Qian, Li Yujin, Zhan Jiajun, Jiang Yuting, Liu Man, Chen Mei, Hu Yueqiang, Huang Ke-Jing, Yang Hongfen, Chen Zhuo, Cai Ren, Tan Weihong
Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio-Sensing and Chemometrics, College of Material Science and Engineering, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China.
Hunan Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China.
ACS Nano. 2025 Apr 22;19(15):15069-15080. doi: 10.1021/acsnano.5c01864. Epub 2025 Apr 11.
In this work, a multifunctional Pd@Au nanoframe hydrogel was designed to detect uric acid (UA) for in situ monitoring of wound infection and enhance wound healing by a chemo-photothermal strategy. In acidic conditions, the Pd@Au nanoframe hydrogels show high peroxidase-like activity by catalyzing HO to produce reactive oxygen species (ROS) to damage RNAs of bacteria and enhance antibacterial activity. Under Near-infrared (NIR) laser irradiation, the Pd@Au nanoframe hydrogels exhibit photothermal conversion performance; i.e., the color of Pd@Au nanoframe hydrogel solution varies from deep blue (0 s, 25.4 °C) to red (300 s, 50.1 °C) in infrared thermography. After loading the antibacterial mupirocin (M), the as-obtained M Pd@Au nanoframe hydrogels show a maximum cumulative release rate exceeding 90% for mupirocin, as controlled by NIR laser irradiation. In antimicrobial experiments in vitro, M Pd@Au nanoframe hydrogels exhibit NIR laser-driven antibacterial ability; i.e., 98% are effectively killed in 10 min. After coating rabbit wounds with a UA sensing patch of M Pd@Au nanoframe hydrogels, wound status can be monitored in real time by detecting UA concentration, leading to rapid wound healing in 4 days by a new synergistic effect of chemo-photothermal strategy. This approach successfully confirms a closed-loop strategy, i.e., real-time monitoring the status of a wound and efficiently perform chemo-photothermal wound therapy, for wound healing by combining functional hydrogels, NIR laser irradiation, and pharmaceutical antibacterials.
在这项工作中，设计了一种多功能Pd@Au纳米框架水凝胶，用于检测尿酸（UA）以原位监测伤口感染，并通过化学-光热策略促进伤口愈合。在酸性条件下，Pd@Au纳米框架水凝胶通过催化HO产生活性氧（ROS）以破坏细菌的RNA并增强抗菌活性，从而表现出高类过氧化物酶活性。在近红外（NIR）激光照射下，Pd@Au纳米框架水凝胶表现出光热转换性能；即在红外热成像中，Pd@Au纳米框架水凝胶溶液的颜色从深蓝色（0秒，25.4℃）变为红色（300秒，50.1℃）。负载抗菌莫匹罗星（M）后，所得到的M-Pd@Au纳米框架水凝胶在近红外激光照射控制下，莫匹罗星的最大累积释放率超过90%。在体外抗菌实验中，M-Pd@Au纳米框架水凝胶表现出近红外激光驱动的抗菌能力；即10分钟内98%的细菌被有效杀灭。用M-Pd@Au纳米框架水凝胶的UA传感贴片覆盖兔伤口后，通过检测UA浓度可实时监测伤口状态，通过化学-光热策略的新协同效应，4天内伤口快速愈合。这种方法成功证实了一种闭环策略，即通过结合功能性水凝胶、近红外激光照射和药物抗菌剂，实时监测伤口状态并有效地进行化学-光热伤口治疗，以实现伤口愈合。
