Melo Bruno Pereira, Oliveira Joyce Camilla Cruz de, Zacarias Aline Cruz, Cordeiro Letícia Maria de Souza, Rodrigues João Gabriel da Silveira, Santos Mara Lívia Dos, Avelar Gleide Fernandes de, Meeusen Romain, Heyman Elsa, Gomes Camila Berbert, Ogando Pedro Henrique Madureira, Soares Danusa Dias
Universidade Federal de Minas Gerais, Departamento de Educação Física, Laboratório de Fisiologia do Exercício, Belo Horizonte, MG, Brasil.
Centro Universitário ITOP (UNITOP) - Instituto Tocantinense de Ensino Superior e Pesquisa Ltda., Palmas, TO, Brasil.
Arch Endocrinol Metab. 2025 Apr 11;68:e240169. doi: 10.20945/2359-4292-2024-0169.
This study investigated the acute effects of cocoa flavanol (CF) supplementation on glucose homeostasis, aerobic performance, and lactate concentration in rats with type 1 diabetes mellitus (T1DM), type 2 diabetes mellitus (T2DM), and normoglycemia (NORM).
The study included 28 male Wistar rats (220-290 g). Induction of T1DM (n = 8) was achieved through intraperitoneal injection of streptozotocin, while T2DM (n = 10) was induced using an ad libitum high-fat diet combined with a fructose-rich beverage. The rats in the NORM group (n = 10) received a standard diet for 30 days. Two experiments were conducted: (1) T1DM rats performed two successive 30-minute treadmill runs below the anaerobic threshold and (2) T2DM and NORM rats underwent two incremental maximal treadmill running tests, both after CF or placebo supplementation. Blood glucose concentrations were measured from pre-exercise to 60 minutes post-exercise.
Glycemic reduction at 60 minutes post-exercise was significantly potentiated by CF compared with placebo supplementation in T1DM, T2DM, and normoglycemic rats (p < 0.05 for all). In T2DM rats, CF induced a glycemic response comparable to the NORM placebo-supplemented condition. These effects of CF persisted despite variations in aerobic performance or lactate concentration after incremental exercise.
Supplementation with CF prior to physical exercise elicited a pronounced post-aerobic exercise glycemic reduction. This represents a promising strategy for mitigating the duration of hyperglycemia exposure after physical exercise.
本研究调查了补充可可黄烷醇（CF）对1型糖尿病（T1DM）、2型糖尿病（T2DM）和血糖正常（NORM）大鼠的葡萄糖稳态、有氧运动能力和乳酸浓度的急性影响。
本研究纳入28只雄性Wistar大鼠（体重220 - 290克）。通过腹腔注射链脲佐菌素诱导T1DM（n = 8），而T2DM（n = 10）则通过自由采食高脂饮食并结合富含果糖的饮料诱导。NORM组（n = 10）的大鼠接受标准饮食30天。进行了两项实验：（1）T1DM大鼠在无氧阈值以下连续进行两次30分钟的跑步机跑步；（2）T2DM和NORM大鼠在补充CF或安慰剂后进行两次递增式最大跑步机跑步测试。在运动前至运动后60分钟测量血糖浓度。
与补充安慰剂相比，CF在T1DM、T2DM和血糖正常的大鼠中显著增强了运动后60分钟的血糖降低效果（所有p < 0.05）。在T2DM大鼠中，CF诱导的血糖反应与补充安慰剂的NORM组相当。尽管递增运动后有氧运动能力或乳酸浓度有所变化，但CF的这些作用仍然持续存在。
在体育锻炼前补充CF可引起有氧运动后明显的血糖降低。这是一种有前景的策略，可减轻体育锻炼后高血糖暴露的持续时间。
