Tu Jianqiu, Liu Xiaoyuan, Li Kun, Liu Huiping, Li Jianfang, Zhu Jing, Xia Nan, Wang Qing
Xinyang Agriculture and Forestry University, Research Center for Comprehensive Utilization of Food Resources of Ta-pieh Mountains, Comprehensive Utilization and Development Key Laboratory in Characteristic Food Resources, Xinyang, Xinyang City, Henan Province, 464000, PR China.
Xinyang Agriculture and Forestry University, Research Center for Comprehensive Utilization of Food Resources of Ta-pieh Mountains, Comprehensive Utilization and Development Key Laboratory in Characteristic Food Resources, Xinyang, Xinyang City, Henan Province, 464000, PR China.
J Ethnopharmacol. 2025 May 12;347:119725. doi: 10.1016/j.jep.2025.119725. Epub 2025 Apr 9.
A novel cold-water-soluble polysaccharide (CALP-1-1) was isolated and purified from Citrus aurantium L. Besides determining its in vitro and in vivo anti-tumor activities, its structure was characterised. The results reveal that CALP-1-1 mainly consists of Rha, Ara, Gal, GalA, and GlcA (molar ratio, 1:14.56:19.27:2.27:1.29) with three main linkages. Its average molecular weight was 2.04 × 10 kDa. Moreover, the triple helix structure of CALP-1-1 was proved by Congo-red and circular dichroism (CD). The in vitro experimental results demonstrate that CALP-1-1 significantly inhibited the proliferation of HepG2 cells with typical apoptotic features by inducing cell cycle arrest in the S phase. Furthermore, in vivo anti-tumor experiments suggest that CALP-1-1 could induce H22 solid tumor cells apoptosis and exhibit anti-tumor effects by protecting immune organs and intensifying the secretion of immune cells (macrophages, lymphocytes and NK cells). In conclusion, CALP-1-1 might be a promising component for cancer treatment.
从酸橙中分离纯化出一种新型冷水溶性多糖（CALP-1-1）。除了测定其体外和体内抗肿瘤活性外，还对其结构进行了表征。结果表明，CALP-1-1主要由鼠李糖、阿拉伯糖、半乳糖、半乳糖醛酸和葡萄糖醛酸组成（摩尔比为1:14.56:19.27:2.27:1.29），有三种主要连接方式。其平均分子量为2.04×10 kDa。此外，通过刚果红和圆二色性（CD）证明了CALP-1-1的三螺旋结构。体外实验结果表明，CALP-1-1通过诱导细胞周期停滞在S期，显著抑制HepG2细胞增殖并具有典型凋亡特征。此外，体内抗肿瘤实验表明，CALP-1-1可诱导H22实体瘤细胞凋亡，并通过保护免疫器官和增强免疫细胞（巨噬细胞、淋巴细胞和NK细胞）分泌发挥抗肿瘤作用。总之，CALP-1-1可能是一种有前途的癌症治疗成分。
