Ibragimov A N, Kyrchanova O V, Vorontsova Y E, Kozlov E N, Dubrovskaya V O, Georgiev P G
Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia.
Dokl Biochem Biophys. 2025 Apr;521(1):183-186. doi: 10.1134/S1607672924601513. Epub 2025 Apr 11.
The autonomous functioning of regulatory domains in the Bithorax complex (BX-C) of Drosophila melanogaster is maintained by boundaries (insulators) that prevent inappropriate enhancer-promoter interactions. Polycomb response elements (PREs) maintain epigenetic memory within regulatory domains and are often located near insulators, enhancing their blocking activity. The bxd PRE is a well-characterized silencer that regulates the bxd/pbx domain of the Ubx gene in the first abdominal segment. We used a boundary replacement strategy to assess the insulator activity of the bxd PRE. Substituting the bxd PRE for the Fab-7 boundary, which separates the Abd-B regulatory domains in the BX-C, revealed that the bxd PRE has weak insulator activity.
果蝇黑腹果蝇双胸复合体（BX-C）中调控结构域的自主功能由边界（绝缘子）维持，这些边界可防止不适当的增强子-启动子相互作用。多梳反应元件（PREs）在调控结构域内维持表观遗传记忆，并且通常位于绝缘子附近，增强其阻断活性。bxd PRE是一个特征明确的沉默子，它调控第一腹节中Ubx基因的bxd/pbx结构域。我们使用边界替换策略来评估bxd PRE的绝缘子活性。将bxd PRE替换为Fab-7边界（该边界分隔BX-C中的Abd-B调控结构域），结果表明bxd PRE具有较弱的绝缘子活性。
