Sadullahoglu Canan, Yaprak Neslihan, Yazısız Veli, Ozbudak İrem Hicran
Department of Pathology, Antalya Training and Research Hospital, 07100 Antalya, Turkey.
Department of Otorhinolaryngology, Antalya City Hospital, 07080 Antalya, Turkey.
J Clin Med. 2025 Mar 31;14(7):2390. doi: 10.3390/jcm14072390.
Primary Sjogren's syndrome (pSS) is an autoimmune disease caused by chronic inflammation of the exocrine glands. We aimed to determine the immunohistochemical parameters that may help in the diagnosis of pSS and to determine histopathological markers for the early diagnosis of patients. Hematoxylin-eosin-stained preparations of salivary gland tissue samples of a control group consisting of 9 healthy patients and 12 patients diagnosed with non-specific chronic sialadenitis (NSCS) and a study group consisting of 39 patients diagnosed with pSS were evaluated. SSA/Ro (TRIM21), SSB/la, BAFF, enolase (ENO1), and MMP-9 antibodies were applied to the samples to assess the presence of staining in the ductal, acini, and inflammation regions. In our study, mild staining with the SSA/Ro (TRIM21) antibody was observed in the ductal areas of all pSS cases, while no staining was detected in the healthy and NSCS cases ( < 0.01). Additionally, stronger staining was identified in the ductal and inflammatory areas of the pSS cases with BAFF compared to the control group. The staining for the ENO1 antibody was higher and more intense in the NSCS and pSS cases compared to those with normal salivary glands, and this difference was found to be statistically significant ( < 0.01). While mild staining was detected in the ductal areas with MMP-9 antibody in most of the NSCS and pSS cases, it was detected in 11% of the healthy cases. : Our study suggests that SSA/Ro (TRIM21), ENO1, MMP9, and BAFF can be used to confirm the diagnosis in cases of suspicion.
原发性干燥综合征（pSS）是一种由外分泌腺慢性炎症引起的自身免疫性疾病。我们旨在确定有助于pSS诊断的免疫组化参数，并确定患者早期诊断的组织病理学标志物。对由9名健康患者和12名诊断为非特异性慢性涎腺炎（NSCS）的患者组成的对照组以及由39名诊断为pSS的患者组成的研究组的唾液腺组织样本苏木精-伊红染色制剂进行评估。将SSA/Ro（TRIM21）、SSB/la、BAFF、烯醇化酶（ENO1）和基质金属蛋白酶-9抗体应用于样本，以评估导管、腺泡和炎症区域的染色情况。在我们的研究中，所有pSS病例的导管区域均观察到SSA/Ro（TRIM21）抗体轻度染色，而健康和NSCS病例中未检测到染色（P<0.01）。此外，与对照组相比，pSS病例的导管和炎症区域中BAFF染色更强。与正常唾液腺相比，NSCS和pSS病例中ENO1抗体的染色更高且更强烈，并且发现这种差异具有统计学意义（P<0.01）。在大多数NSCS和pSS病例中，MMP-9抗体在导管区域检测到轻度染色，而在11%的健康病例中检测到。我们的研究表明，SSA/Ro（TRIM21）、ENO1、MMP9和BAFF可用于疑似病例的诊断确认。
