Kouda M, Ouchi Y, Takasaki Y, Maeda T, Matsuzaki H, Nakaya R
Microbiol Immunol. 1985;29(4):309-15. doi: 10.1111/j.1348-0421.1985.tb00829.x.
A method of rapid susceptibility testing by bioluminescent assay was developed. Correlation between the 50% inhibition dose of antimicrobics for bacterial adenosine triphosphate measured by bioluminescent assay and the minimum inhibitory concentration obtained by the broth dilution method was satisfactory. In the bioluminescent assay the incubation time required was only 90 min.
开发了一种通过生物发光测定法进行快速药敏试验的方法。通过生物发光测定法测得的抗菌药物对细菌三磷酸腺苷的50%抑制剂量与肉汤稀释法获得的最低抑菌浓度之间的相关性良好。在生物发光测定法中,所需的孵育时间仅为90分钟。