Takte Mahesh A, Patil Shubham S, Fulari Akash V, Hianik Tibor, Shirsat Mahendra D
RUSA Center for Advanced Sensor Technology, Department of Physics, Dr. Babasaheb Ambedkar Marathwada University, Chhatrapati Sambhajinagar 431004, MS, India.
Symbiosis Centre for Nanoscience and Nanotechnology, Symbiosis International (Deemed University), Pune 412115, MS, India.
Sensors (Basel). 2025 Apr 7;25(7):2334. doi: 10.3390/s25072334.
We developed a sensor consisting of VO nanorods and a reduced graphene oxide (rGO) nanocomposite (VO/rGO) with immobilized DNA aptamers (Apt-NH@VO/rGO) for the sensitive electrochemical detection of Hg (II). The VO nanorods anchored on rGO nanosheets were synthesized using a hydrothermal method. The nanocomposite was analyzed by various powerful physical methods that include X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), field emission scanning electron microscopy (FE-SEM), Raman spectroscopy, the Brunauer-Emmett-Teller (BET) method, and Fourier transform infrared spectroscopy (FTIR). The FE-SEM of VO disclosed the nanorod-like structure and uniform anchoring of VO on the rGO nanosheet. Moreover, the BET results showed that the VO/rGO nanocomposite possesses excellent porosity. Furthermore, a glassy carbon electrode (GCE) was modified with Apt-NH@VO/rGO and used for the electrochemical detection of Hg(II) by differential pulse voltammetry (DPV). The aptasensor exhibited excellent sensitivity and selectivity toward Hg(II) detection, with a limit of detection (LOD) of 5.57 nM, which is below the maximum permissible limit established by WHO for rivers (30 nM). The sensor also exhibited significant stability and good repeatability.
我们开发了一种由VO纳米棒和还原氧化石墨烯（rGO）纳米复合材料（VO/rGO）组成的传感器，该复合材料固定有DNA适体（Apt-NH@VO/rGO），用于对Hg（II）进行灵敏的电化学检测。采用水热法合成了锚定在rGO纳米片上的VO纳米棒。通过多种强大的物理方法对该纳米复合材料进行了分析，这些方法包括X射线衍射（XRD）、能量色散X射线光谱（EDX）、场发射扫描电子显微镜（FE-SEM）、拉曼光谱、布鲁瑙尔-埃米特-特勒（BET）法和傅里叶变换红外光谱（FTIR）。VO的FE-SEM显示出纳米棒状结构以及VO在rGO纳米片上的均匀锚定。此外，BET结果表明VO/rGO纳米复合材料具有优异的孔隙率。此外，用Apt-NH@VO/rGO修饰玻碳电极（GCE），并用于通过差分脉冲伏安法（DPV）对Hg（II）进行电化学检测。该适体传感器对Hg（II）检测表现出优异的灵敏度和选择性，检测限（LOD）为5.57 nM，低于世界卫生组织规定的河流最大允许限值（30 nM）。该传感器还表现出显著的稳定性和良好的重复性。
