Polo Ana Caroline, Uber Thaís Marques, Souza Gustavo Henrique, Corrêa Rúbia Carvalho Gomes, Dos Santos Filho José Rivaldo, de Sá-Nakanishi Anacharis Babeto, Seixas Flávio Augusto Vicente, Bracht Adelar, Peralta Rosane Marina
Postgraduate Program in Food Science, State University of Maringá, Maringa 87020-900, PR, Brazil.
Postgraduate Program in Biochemistry, State University of Maringá, Maringa 87020-900, PR, Brazil.
Plants (Basel). 2025 Apr 5;14(7):1133. doi: 10.3390/plants14071133.
Jatobá () is a native tree abundant in Brazil. The fruit coat is an industrial by-product of jatobá flour processing, typically discarded. Presently, within the circular bioeconomy concept, there are efforts underway that aim at finding economically viable applications for the bio-residues of jatobá. Within this context, the present work attempts to find possible applications for the jatobá coat in glycemic control through inhibition of α-amylase activity. Aqueous and hydroethanolic extracts were used. In vitro experiments included detailed kinetic studies with an α-amylase catalyzed reaction. Starch absorption in vivo was assessed by means of a starch tolerance test in mice. Both extracts inhibited α-amylase. The IC values for the aqueous and hydroalcoholic extracts were 81.98 ± 3.53 µg/mL and 51.06 ± 0.42 µg/mL, respectively. The inhibition was of the non-competitive type. Both extracts reduced hyperglycemia caused by starch administration in mice, the aqueous extract being effective over a larger dose range. This action can be attributed to the α-amylase inhibition. In silico studies suggested that procyanidin dimers, taxifolin 7-O-rhamnoside, and quercetin 7-rhamnoside contribute, but several other not-yet-identified substances may be involved. The findings suggest that aqueous and hydroalcoholic extracts from jatobá coat warrant further investigations as potential modulators of glycemia following starch ingestion.
jatobá（）是一种在巴西大量生长的本土树木。其果皮是jatobá面粉加工的工业副产品，通常被丢弃。目前，在循环生物经济概念的框架下，人们正在努力为jatobá的生物残渣寻找经济可行的应用。在此背景下，本研究试图通过抑制α-淀粉酶活性来探寻jatobá果皮在血糖控制方面的潜在应用。使用了水提取物和水乙醇提取物。体外实验包括对α-淀粉酶催化反应进行详细的动力学研究。通过小鼠淀粉耐量试验评估体内淀粉吸收情况。两种提取物均能抑制α-淀粉酶。水提取物和水乙醇提取物的半数抑制浓度（IC）值分别为81.98±3.53μg/mL和51.06±0.42μg/mL。抑制作用为非竞争性类型。两种提取物均能降低小鼠因摄入淀粉引起的高血糖，水提取物在更大剂量范围内有效。这种作用可归因于对α-淀粉酶的抑制。计算机模拟研究表明，原花青素二聚体、taxifolin 7-O-鼠李糖苷和槲皮素7-鼠李糖苷起了作用，但可能还涉及其他几种尚未确定的物质。研究结果表明，jatobá果皮的水提取物和水乙醇提取物作为淀粉摄入后血糖潜在调节剂值得进一步研究。
