Urdapilleta Mara, Pech-May Angélica, Lamattina Daniela, Burgos Eliana Florencia, Gabriela Giuliani Magalí, Cortés María Micaela, Espinoza-Carniglia Mario, Salomón Oscar Daniel, Lareschi Marcela
Instituto Nacional de Medicina Tropical-Administración Nacional de Laboratorios e Institutos (INMeT-ANLIS), Puerto Iguazú, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina.
Med Vet Entomol. 2025 Sep;39(3):445-455. doi: 10.1111/mve.12801. Epub 2025 Apr 12.
The aim of this study was to analyse the prevalence and distribution of Bartonella species in companion animals, wildlife and their associated fleas in different landscapes of the Atlantic Forest ecoregion of Argentina, and to analyse the phylogenetic relationships between the Bartonella species identified in this study in addition to those found in other regions available in GenBank. A total of 257 mammals, including rodents, opossums, dogs and cats, were examined to detect the presence of Bartonella in both fleas and host tissues. The study revealed the presence of Bartonella in rodent fleas (47.7%), opossum fleas (7.8%), rodent spleens (6.5%) and cat blood (10%). Analysis of 15 gltA gene fragments revealed the existence of at least three different genotypes and eight haplotypes. Genetic analysis has established a close association between Bartonella species from mammals and fleas in both the Atlantic Forest of Brazil and the Costanera Sur Reserve in Argentina. Bartonella henselae (Regnery et al.) Brenner et al. (Hyphomicrobiales: Bartonellaceae) was identified in three rodent species: Rattus rattus (L.; Muridae), Akodon montensis Thomas (Cricetidae) and Calomys tener Winge (Cricetidae). This marks the first report of B. henselae in R. rattus in Argentina, as well as the initial documentation of its association with A. montensis and C. tener. Additionally, Bartonella clarridgeiae Lawson and Collins (Hyphomicrobiales: Bartonellaceae) was identified in feline blood and in fleas from dogs and cats. These findings are particularly relevant in the context of areas undergoing deforestation, where interactions between wild and domestic hosts increase the risk of zoonotic disease transmission. A One Health approach is crucial to understand transmission dynamics and to address the challenges posed by fleas as infection amplifiers and spillover phenomena. These present significant challenges for public health and biodiversity conservation.
本研究的目的是分析阿根廷大西洋森林生态区不同景观中伴侣动物、野生动物及其相关跳蚤中巴通体属物种的流行情况和分布，并分析本研究中鉴定出的巴通体属物种与基因库中其他地区发现的物种之间的系统发育关系。共检查了257只哺乳动物，包括啮齿动物、负鼠、狗和猫，以检测跳蚤和宿主组织中是否存在巴通体。研究发现啮齿动物跳蚤（47.7%）、负鼠跳蚤（7.8%）、啮齿动物脾脏（6.5%）和猫血液（10%）中存在巴通体。对15个gltA基因片段的分析揭示了至少三种不同基因型和八种单倍型的存在。遗传分析表明，巴西大西洋森林和阿根廷科斯坦内拉苏尔保护区的哺乳动物和跳蚤中的巴通体属物种之间存在密切关联。在三种啮齿动物物种中鉴定出了亨氏巴通体（Regnery等人）Brenner等人（丝状杆菌目：巴通体科）：黑家鼠（L.；鼠科）、山地稻鼠托马斯（仓鼠科）和细毛棉鼠温格（仓鼠科）。这是阿根廷黑家鼠中亨氏巴通体的首次报告，也是其与山地稻鼠和细毛棉鼠关联的首次记录。此外，在猫血液以及狗和猫身上的跳蚤中鉴定出了克拉氏巴通体劳森和柯林斯（丝状杆菌目：巴通体科）。在森林砍伐地区，这些发现尤为重要，因为野生宿主和家养宿主之间的相互作用增加了人畜共患病传播的风险。一种“同一健康”方法对于理解传播动态以及应对跳蚤作为感染放大器和溢出现象所带来的挑战至关重要。这些对公共卫生和生物多样性保护构成了重大挑战。
