Ishii Yasuto, Toyoda Atsushi, Lewis Alec, Davison Angus, Miura Osamu, Kimura Kazuki, Chiba Satoshi
Graduate School of Life Sciences, Tohoku University, Sendai, Japan.
Advanced Genomics Center, National Institute of Genetics, Mishima, Japan.
Genome Biol Evol. 2025 Apr 30;17(5). doi: 10.1093/gbe/evaf070.
While terrestrial land snails have long been used for evolutionary research, a lack of high-quality genomic resources has impeded recent progress. Bradybaena snails in particular have numerous intriguing traits that make them a good model for studying evolution, including shell pattern polymorphism and convergent evolution. They are also introduced and invasive across the world. In this study, we present a chromosome-level genome assembly of the Asian tramp snail Bradybaena similaris, utilizing 88-fold Illumina short-read sequences, 125-fold Nanopore long-read sequences, 63-fold PacBio HiFi sequences, and 47-fold Hi-C sequences. The assembled genome of 2.18 Gb is anchored to 28 chromosomes and exhibits high completeness (single copy, 91.7%; duplicates, 7.1%) and contiguity (N50 of 75.6 Mb). Additionally, we also obtained a high-quality transcriptome for annotation. This resource represents the first chromosome-level assembly for snails in the superfamily Helicoidea, which includes more than 5,000 species of terrestrial snails, and will facilitate genomic study in Bradybaena and, more broadly, in the superfamily Helicoidea.
虽然陆生蜗牛长期以来一直被用于进化研究，但缺乏高质量的基因组资源阻碍了近期的进展。特别是巴蜗牛具有许多有趣的特征，使其成为研究进化的良好模型，包括壳纹多态性和趋同进化。它们在世界各地也是外来入侵物种。在本研究中，我们利用88倍的Illumina短读长序列、125倍的Nanopore长读长序列、63倍的PacBio HiFi序列和47倍的Hi-C序列，展示了亚洲入侵蜗牛相似巴蜗牛的染色体水平基因组组装。组装的2.18 Gb基因组被锚定到28条染色体上，具有高完整性（单拷贝，91.7%；重复序列，7.1%）和连续性（N50为75.6 Mb）。此外，我们还获得了用于注释的高质量转录组。该资源代表了柄眼超科蜗牛的第一个染色体水平组装，柄眼超科包括5000多种陆生蜗牛，将促进巴蜗牛以及更广泛的柄眼超科的基因组研究。
