Carrasco-Juan José-Luis, Tapia Olga, González-Gómez Miriam, Vega-Falcón Abian, García-Hernández Sonia, Rufino-Gómez Alexis, Méndez-Medina Rafael, Álvarez-Arguelles Cabrera Hugo
Área de Histología, Departamento de Ciencias Médicas Básicas, Grado en Medicina, Facultad de Ciencias de La Salud, Universidad de La Laguna, C/Santa María Soledad s/n, Apartado 456, 38200, Santa Cruz de Tenerife, Islas Canarias, Spain.
Instituto de Tecnologías Biomédicas, Departamento de Ciencias Médicas Básicas, Grado en Medicina, Facultad de Ciencias de La Salud, Universidad de La Laguna, C/Santa María Soledad s/n, Apartado 456, 38200, Santa Cruz de Tenerife, Islas Canarias, Spain.
Hum Cell. 2025 Apr 12;38(3):85. doi: 10.1007/s13577-025-01210-1.
In our search for markers to identify and study ovarian Leydig cells, we utilized immunohistochemical techniques and visualized the results using conventional and confocal microscopy. We successfully employed steroidogenic factor- 1 (SF1), androgen receptor (AR), and class III β-tubulin as markers. SF1 and AR specifically highlighted the intraneural cell precursors of Leydig cells, which were previously identified in a published case of mature cystic teratoma of the ovary, and the adult ovarian Leydig cells. Furthermore, the transient expression of class III β-tubulin could be associated with the intraneural displacement of these precursors, cooperating in their migration to colonize the ovaries of adult women.
在寻找用于鉴定和研究卵巢莱迪希细胞的标志物的过程中，我们采用了免疫组织化学技术，并使用传统显微镜和共聚焦显微镜观察结果。我们成功地将类固醇生成因子-1（SF1）、雄激素受体（AR）和III类β-微管蛋白用作标志物。SF1和AR特别突出了莱迪希细胞的神经内细胞前体，这些前体先前在一篇已发表的卵巢成熟囊性畸胎瘤病例中被鉴定出来，以及成年卵巢莱迪希细胞。此外，III类β-微管蛋白的瞬时表达可能与这些前体的神经内移位有关，协同它们迁移以在成年女性卵巢中定植。
