通过跨突触绿色荧光蛋白互补监测突触

Monitoring Synapses via Trans-synaptic GFP Complementation.

作者信息

Tsetsenis Theodoros

机构信息

Division of Neurology, Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA, USA.

出版信息

Methods Mol Biol. 2025;2910:127-134. doi: 10.1007/978-1-0716-4446-1_8.

DOI:10.1007/978-1-0716-4446-1_8

PMID:40220097

Abstract

Over the last years, the analysis of synaptic connectivity in the mammalian brain has been accelerated by the use of techniques combining electrophysiology, light microscopy, viral tracing, and genetic manipulations in animal models. Of particular interest are methods that aim to label synapses by tethering complementary split GFP fragments in opposing sites of the synaptic cleft. Here, I describe SynView, a method for monitoring synapse formation based on GFP complementation and provide a detailed protocol for use in neuronal cultures from mouse hippocampus.

摘要

在过去几年中，结合电生理学、光学显微镜、病毒示踪和动物模型中的基因操作等技术，加速了对哺乳动物大脑中突触连接性的分析。特别令人感兴趣的是那些旨在通过在突触间隙的相对位点连接互补的分裂绿色荧光蛋白（GFP）片段来标记突触的方法。在此，我描述了SynView，一种基于GFP互补监测突触形成的方法，并提供了在小鼠海马体神经元培养物中使用的详细方案。