In Situ Assessment of Potentially Toxic Elements (PETs) in Different Tissues of Leopard Frogs (Lithobates berlandieri) Residing Near a Mining Site in Charcas, San Luis Potosi, Mexico.

Mining generates large volumes of waste, which, if not regulated, can release toxic metals, causing widespread environmental contamination. This study focused on heavy metal contamination in sediments and amphibians within a mining area at Charcas, San Luis Potosi, Mexico. The results revealed that the Lithobates berlandieri individuals inhabiting an area polluted with industrial waste, high levels of heavy metals and metalloids (As, Cd, Cr, Cu, Hg and Pb) were found in sediments and tissues compared to samples from a reference site. Furthermore, the levels of all metals and metalloids in these frogs were higher in the kidney, liver, and skin. Additionally, it can contribute to the proposed use of non-lethal biomarkers (e.g. skin) for medium and long-term monitoring and regulation at these sites.