Choe Hyung Woon, Kim DanA, Choi Young Jun, Koh Hyun Gi, Park Won-Kun
Department of Chemistry, Sangmyung University, Seoul 03016, Republic of Korea.
Department of Biological Engineering, Konkuk University, Seoul 05029, Republic of Korea.
J Microbiol Biotechnol. 2025 Apr 9;35:e2412049. doi: 10.4014/jmb.2412.12049.
As the importance of achieving a carbon-neutral society grow, research on the production of microalgal bioproducts have gained significant attention. Among the production processes, cultivation has emerged as a critical step as it determines overall productivity and feasibility. However, changes in cell properties during cultivation pose challenges, and there is currently no direct method to simultaneously assess cell growth, cellular division properties and cell enlargement. In this study, two green algae ( sp. ABC001 and sp. HS2) were cultivated under photo and mixotrophic conditions to evaluate their growth, cell size profile, and changes in optical density. The mixotrophic condition resulted in 2.1 and 1.6-fold higher biomass yields than the photoautotrophic condition for ABC001 and HS2, respectively at day 4. Over the 4-day cultivation, cell sizes ranged from 2.51 to 5.57 μm for ABC001 and from 2.56 to 4.41 μm for HS2. By analyzing changes in the conversion factor between dry cell weight (DCW) and optical density (OD), it was observed that variations in the slope correlated with changes in cell size. Additionally, chlorophyll content fluctuated during cultivation, reaching maximum levels (14.34 and 16.58 μg/mg biomass, respectively) under phototrophic condition on day 2. This study highlights the relationship between cell division tendencies, DCW, cell size, and OD, demonstrating their critical role in determining cellular component content. For future optimization of algal product production processes, further research on these cellular differentiation mechanisms will be essential.
随着实现碳中和社会的重要性日益增加，微藻生物产品生产的研究受到了广泛关注。在生产过程中，培养已成为关键步骤，因为它决定了整体生产力和可行性。然而，培养过程中细胞特性的变化带来了挑战，目前尚无直接方法可同时评估细胞生长、细胞分裂特性和细胞增大情况。在本研究中，两种绿藻（ABC001种和HS2种）在光照和混合营养条件下进行培养，以评估它们的生长、细胞大小分布和光密度变化。在第4天，混合营养条件下ABC001和HS2的生物量产量分别比光合自养条件高出2.1倍和1.6倍。在为期4天的培养过程中，ABC001的细胞大小范围为2.51至5.57μm，HS2的细胞大小范围为2.56至4.41μm。通过分析干细胞重量（DCW）与光密度（OD）之间转换因子的变化，发现斜率的变化与细胞大小的变化相关。此外，叶绿素含量在培养过程中波动，在第2天的光养条件下达到最高水平（分别为14.34和16.58μg/mg生物量）。本研究突出了细胞分裂趋势、DCW、细胞大小和OD之间的关系，证明了它们在确定细胞成分含量方面的关键作用。为了未来优化藻类产品生产工艺，对这些细胞分化机制进行进一步研究至关重要。
