Fereydouni Tara, Zargar Seyed Jalal, Seifi Sharareh, Sheikhpour Mojgan
Department of Biology, Alborz Campus, University of Tehran, Tehran, Iran.
Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Iran Biomed J. 2025 Jan 1;29(1 & 2):49-56. doi: 10.61186/ibj.4354.
In non-small cell lung cancer (NSCLC), miR-200a plays a significant role in apoptosis. One of the genes involved in this pathway is XIAP, which has been shown anti-apoptotic activity. Research has indicated a significant association between miR-200a and the XIAP gene in this pathway. The present study investigated the expression profiles of miR-200a and the XIAP gene in NSCLC patients compared to normal individuals, as well as cancer cells compared to normal and apoptosis-inducing conditions.
In this study, 40 blood specimens were collected from NSCLC patients and 40 from healthy individuals. After isolating plasma and peripheral blood mononuclear cells from these samples, we analyzed the miR-200a and XIAP gene expression levels using real-time PCR. Subsequently, normal and lung cancer cells were treated with paclitaxel as a model of apoptosis. The antiproliferative effects and induction of apoptosis were then evaluated using the MTT and flow cytometry assays, respectively. Finally, the expression patterns of miR-200a and the XIAP gene were investigated through a real-time PCR method.
Results indicated that the miR-200a expression level was lower in NSCLC patients than in healthy ones, while the expression level of XIAP gene increased in the NSCLC Patients’ blood. The MTT and flow cytometry results demonstrated a decreased proliferation and increased apoptosis rates in two lung line cells (A549 and MRC5) treated with paclitaxel. XIAP expression level also decreased in A549 cells treated with paclitaxel compared to untreated A549 cells.
MiR-200a may be associated with the XIAP gene expression and the induction of the apoptosis pathway in NSCLC.
在非小细胞肺癌（NSCLC）中，miR-200a在细胞凋亡中起重要作用。该通路涉及的基因之一是XIAP，其具有抗凋亡活性。研究表明，在该通路中miR-200a与XIAP基因之间存在显著关联。本研究调查了NSCLC患者与正常个体相比，以及癌细胞与正常及诱导凋亡条件相比时，miR-200a和XIAP基因的表达谱。
在本研究中，从NSCLC患者中收集了40份血液样本，从健康个体中收集了40份血液样本。从这些样本中分离出血浆和外周血单核细胞后，我们使用实时PCR分析了miR-200a和XIAP基因的表达水平。随后，用紫杉醇处理正常细胞和肺癌细胞作为凋亡模型。然后分别使用MTT和流式细胞术分析评估抗增殖作用和凋亡诱导情况。最后，通过实时PCR方法研究miR-200a和XIAP基因的表达模式。
结果表明，NSCLC患者中miR-200a的表达水平低于健康个体，而XIAP基因在NSCLC患者血液中的表达水平升高。MTT和流式细胞术结果表明，用紫杉醇处理的两种肺癌细胞系（A549和MRC5）的增殖减少，凋亡率增加。与未处理的A549细胞相比，用紫杉醇处理的A549细胞中XIAP表达水平也降低。
MiR-200a可能与NSCLC中XIAP基因表达及凋亡途径的诱导有关。
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