Khamhan Wimonchat, Srisatjaluk Ratchapin Laovanitch, Pudla Matsayapan, Ruangsawasdi Nisarat, Kuphasuk Yosvimol
Residency Training Program in Periodontology, Faculty of Dentistry, Mahidol University, Bangkok, Thailand.
Dental Department, Pranangklao Hospital, Nonthaburi, Thailand.
J Dent Sci. 2025 Apr;20(2):936-942. doi: 10.1016/j.jds.2024.08.019. Epub 2024 Sep 5.
BACKGROUND/PURPOSE: The adjunctive use of antibiotics in periodontal therapy may lead to resistance and undesired effects, which can be mitigated by alternative agent such as . Thus, this study aimed to formulate fibrin hydrogel incorporated with essential oil (LC-EO) and test its antimicrobial property against key periodontal pathogens and cytotoxicity on human gingival fibroblasts (HGFs).
Fibrin hydrogels were incorporated with various concentrations of LC-EO and the releasing of LC-EO was determined by measuring the wavelength absorbance. Their antibacterial activity was tested against and by the direct contact method. The cytotoxicity to HGFs was determined by MTT assay.
The absorbance wavelength of LC-EO at 230 nm was the optimal wavelength using for the standard curve. The release of 1.0% (v/v) LC-EO from fibrin hydrogel in PBS was 0.03% (v/v) in the first hour and lasted for 24 h. The gradually release of LC-EO was observed until day 30 with the concentration of 0.008% (v/v). The minimum bactericidal concentration against and was 1.0 % (v/v) and 0.25% (v/v), respectively. The lowest cytotoxicity to HGF cells was observed at 0.0625% (v/v), yielding the cell viability of 73.65 ± 8.24 % at 24 h.
Fibrin hydrogel with LC-EO effectively inhibited bacterial growth in a dose-dependent manner. Cytotoxicity assays also revealed concentration-dependent effects on HGF. Our results suggest the potential use of fibrin hydrogel with LC-EO as a novel adjunctive treatment in periodontal diseases.
背景/目的：在牙周治疗中辅助使用抗生素可能会导致耐药性和不良影响，而诸如……等替代药物可以减轻这些问题。因此，本研究旨在制备含有……精油（LC-EO）的纤维蛋白水凝胶，并测试其对主要牙周病原体的抗菌性能以及对人牙龈成纤维细胞（HGFs）的细胞毒性。
将不同浓度的LC-EO掺入纤维蛋白水凝胶中，并通过测量波长吸光度来测定LC-EO的释放情况。通过直接接触法测试其对……和……的抗菌活性。采用MTT法测定对HGFs的细胞毒性。
LC-EO在230nm处的吸光波长是用于标准曲线的最佳波长。在PBS中，1.0%（v/v）LC-EO从纤维蛋白水凝胶中的释放在第1小时为0.03%（v/v），并持续24小时。观察到LC-EO在第30天逐渐释放，浓度为0.008%（v/v）。对……和……的最低杀菌浓度分别为1.0%（v/v）和0.25%（v/v）。在0.0625%（v/v）时观察到对HGF细胞的细胞毒性最低，在24小时时细胞活力为73.65±8.24%。
含有LC-EO的纤维蛋白水凝胶以剂量依赖的方式有效抑制细菌生长。细胞毒性试验也显示了对HGF的浓度依赖性影响。我们的结果表明，含有LC-EO的纤维蛋白水凝胶作为牙周疾病的新型辅助治疗方法具有潜在用途。
