Fujioka-Kobayashi Masako, Schaller Benoit, Pikos Michael A, Sculean Anton, Miron Richard J
Department of Cranio-Maxillofacial Surgery, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, Switzerland.
Private Practice, Pikos Institute, Trinity, FL 34655, USA.
Materials (Basel). 2020 Jul 17;13(14):3190. doi: 10.3390/ma13143190.
Most available antiseptic solutions available today have strong antibacterial effects, however most also possess major cytotoxic effects on human gingival tissues. The VEGA Oral Care Recovery Kit (StellaLife), previously evaluated in clinical studies, consists of 16 active ingredients that are monographed in the Homeopathic Pharmacopeia of United States (HPUS) and recognized for their accelerated healing properties (reduction in post-op pain). The aim of this study was to compare VEGA to chlorhexidine (CHX) in vitro on gingival fibroblast viability, survival at various concentrations, migration assay, proliferation activity, expression of both regenerative growth factors as well as inflammatory markers, and collagen synthesis. A 10-fold dilution of standard CHX (0.02%) led to cell death, whereas cell viability was significantly better in the VEGA group for all tested parameters. Furthermore, VEGA also induced significantly greater fibroblast migration and proliferation. CHX negatively impacted the cellular inflammatory response of gingival fibroblasts, and also led to a reduction in collagen synthesis (50% decrease). Findings from the present study provide support from basic laboratory experiments that validate the previous clinical studies supporting the use of the VEGA oral rinse on its superior biocompatibility and wound healing properties when compared to CHX.
目前市面上大多数现有的抗菌溶液都具有很强的抗菌效果，但大多数也对人体牙龈组织具有主要的细胞毒性作用。VEGA口腔护理恢复套装（StellaLife），此前已在临床研究中进行过评估，它由16种活性成分组成，这些成分在美国顺势疗法药典（HPUS）中有专论，并因其加速愈合特性（减轻术后疼痛）而得到认可。本研究的目的是在体外比较VEGA与氯己定（CHX）对牙龈成纤维细胞活力、不同浓度下的存活率、迁移试验、增殖活性、再生生长因子以及炎症标志物的表达和胶原蛋白合成的影响。标准CHX（0.02%）稀释10倍会导致细胞死亡，而在所有测试参数中，VEGA组的细胞活力明显更好。此外，VEGA还能显著诱导成纤维细胞的迁移和增殖。CHX对牙龈成纤维细胞的细胞炎症反应有负面影响，还导致胶原蛋白合成减少（降低50%）。本研究的结果为基础实验室实验提供了支持，这些实验验证了先前的临床研究，即与CHX相比，VEGA漱口水具有卓越的生物相容性和伤口愈合特性。
