Zhang Jianing, Li Shixuan, Han Yige, Wang Shouchuang, Liu Penghui, Yang Jun
National Key Laboratory for Tropical Crop Breeding, School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, China.
National Key Laboratory for Tropical Crop Breeding, College of Tropical Agriculture and Forestry, Hainan University, Sanya, China.
Bio Protoc. 2025 Apr 5;15(7):e5259. doi: 10.21769/BioProtoc.5259.
Metabolite modifications play a critical role in enhancing plants' adaptability to environmental changes and serve as a major source of functional diversity in metabolites. However, current metabolomics approaches are limited to targeted analyses of a small number of known modified metabolites and lack comprehensive, large-scale studies of plant metabolite modifications. Here, we describe a widely targeted metabolite modificomics (WTMM) strategy, developed using ultra-high-performance liquid chromatography-quadrupole linear ion trap (UHPLC-Q-Trap) and ultra-high-performance liquid chromatography-Q-Exactive Orbitrap (UHPLC-QE-Orbitrap) technologies. This strategy enables high-throughput identification and sensitive quantification of modified metabolites. Using tomato as a model, we conducted a metabolite modificomics study and constructed a WTMM database, identifying 165 novel modified metabolites. The WTMM strategy is broadly applicable and can be extended to the study of other plant species. Key features • WTMM enables large-scale detection and quantitative analysis of plant-modified metabolites. • Integration of UHPLC-Q-Trap and UHPLC-QE-Orbitrap technologies. • The WTMM database is extensible and applicable to other plant species.
代谢物修饰在增强植物对环境变化的适应性方面发挥着关键作用，并且是代谢物功能多样性的主要来源。然而，目前的代谢组学方法仅限于对少数已知修饰代谢物的靶向分析，缺乏对植物代谢物修饰的全面、大规模研究。在此，我们描述了一种广泛靶向代谢物修饰组学（WTMM）策略，该策略是利用超高效液相色谱-四极杆线性离子阱（UHPLC-Q-Trap）和超高效液相色谱-Q-Exactive 轨道阱（UHPLC-QE-Orbitrap）技术开发的。该策略能够对修饰代谢物进行高通量鉴定和灵敏定量。以番茄为模型，我们开展了一项代谢物修饰组学研究并构建了一个 WTMM 数据库，鉴定出 165 种新型修饰代谢物。WTMM 策略具有广泛的适用性，可扩展到其他植物物种的研究。关键特性 • WTMM 能够对植物修饰代谢物进行大规模检测和定量分析。 • 整合了 UHPLC-Q-Trap 和 UHPLC-QE-Orbitrap 技术。 • WTMM 数据库具有可扩展性，适用于其他植物物种。
