School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, 572025, China.
School of Tropical Agriculture and Forestry, Hainan University, Haikou, 572208, China.
J Integr Plant Biol. 2024 Apr;66(4):810-823. doi: 10.1111/jipb.13629. Epub 2024 Feb 20.
The structural and functional diversity of plant metabolites is largely created via chemical modification of a basic backbone. However, metabolite modifications in plants have still not been thoroughly investigated by metabolomics approaches. In this study, a widely targeted metabolite modificomics (WTMM) strategy was developed based on ultra-high performance liquid chromatography-quadrupole-linear ion trap (UHPLC-Q-Trap) and UHPLC-Q-Exactive-Orbitrap (UHPLC-QE-Orbitrap), which greatly improved the detection sensitivity and the efficiency of identification of modified metabolites. A metabolite modificomics study was carried out using tomato as a model, and over 34,000 signals with MS2 information were obtained from approximately 232 neutral loss transitions. Unbiased metabolite profiling was also performed by utilizing high-resolution mass spectrometry data to annotate a total of 2,118 metabolites with 125 modification types; of these, 165 modified metabolites were identified in this study. Next, the WTMM database was used to assess diseased tomato tissues and 29 biomarkers were analyzed. In summary, the WTMM strategy is not only capable of large-scale detection and quantitative analysis of plant-modified metabolites in plants, but also can be used for plant biomarker development.
植物代谢物的结构和功能多样性在很大程度上是通过对基本骨架的化学修饰产生的。然而，代谢组学方法仍未对植物代谢物的修饰进行深入研究。在这项研究中，开发了一种基于超高效液相色谱-四极杆线性离子阱（UHPLC-Q-Trap）和超高效液相色谱-四极杆-Orbitrap（UHPLC-QE-Orbitrap）的广泛靶向代谢物修饰组学（WTMM）策略，该策略大大提高了修饰代谢物的检测灵敏度和鉴定效率。本研究以番茄为模型进行了代谢物修饰组学研究，从大约 232 个中性丢失跃迁中获得了超过 34000 个具有 MS2 信息的信号。通过利用高分辨质谱数据进行无偏代谢物分析，总共注释了 2118 种代谢物和 125 种修饰类型，其中本研究共鉴定出 165 种修饰代谢物。然后，使用 WTMM 数据库评估患病番茄组织，分析了 29 种生物标志物。总之，WTMM 策略不仅能够大规模检测和定量分析植物中的修饰代谢物，还可以用于植物生物标志物的开发。
