Mbankou Sorelle Ngassam, Fokoua Aliance Romain, Koho Cedric Wamba, Foguieng Roger Hermann Sadie, Tabatabaei Sahar Mofidi, Nono Nankam Pamela Arielle, Tidgewell Kevin Joseph, Nguelefack Télesphore Benoît
Research Unit of Animal Physiology and Phytopharmacology, Faculty of Sciences, University of DSchang, Dschang, Cameroon.
Pharmaceutical Sciences Department, University of Kentucky, Lexington, Kentucky 40506, USA.
Adv Pharmacol Pharm Sci. 2025 Feb 28;2025:1244498. doi: 10.1155/adpp/1244498. eCollection 2025.
The pain-depression dyad is highly prevalent and has reciprocal psychological and behavioral effects, leading to poor quality of life, increased disability, and challenging therapeutic outcomes. In an attempt to find better substances that can target pain-depression comorbidity, we examined the effect of aqueous (AE) and ethanol (EE) extracts from () stem bark on reserpinized mice (female and male Swiss albino mice aged 2-3 months). The dyad was induced with 3 injections (Days 1-3) of reserpine (1 mg/kg/day, .). Then, animals were treated (Days 4-8) with plant extracts (25, 50 and 100 mg/kg/day, .) or L-tryptophane (100 mg/kg/day, .). Pain-like (tactile and cold allodynia) and depression-like (pole, tail suspension, and force swimming tests) behavioral parameters were evaluated on Days 4 and 8. On Day 9, animals were sacrificed for the quantification of acetylcholinesterase activity, oxidative stress parameters, total catecholamines, dopamine, serotonin, IL-1β, and TNF-α levels in the brain or spinal cord. IL-1β and TNF-α were also assayed in the serum. The acute toxicity and phytochemical analysis of EE were conducted. Reserpine-induced tactile and cold allodynia, depression-like behavior, increased serum IL-1β and TNF-α, brain acetylcholinesterase activity, and decreased catecholamine concentration were all reversed by AE and EE. Plant extracts significantly increased dopamine levels and reduced oxidative stress in the brain and/or spinal cord. No significant effect was observed on brain serotonin and TNF-α. EE elicited the best pharmacological activity and was nontoxic. LC-MS/MS molecular networking phytochemical analysis identified 5 compounds with high certainty including piperine, aurantiamide acetate, and asperphenamate. AE and EE are effective against pain and depression. Their pharmacological activities might be related to the modulation of inflammation, oxidative stress and catecholamine, and the presence of bioactive natural products.
疼痛-抑郁二元组极为常见，具有相互的心理和行为影响，导致生活质量下降、残疾增加以及治疗效果不佳。为了寻找能够针对疼痛-抑郁共病的更好物质，我们研究了[植物名称]茎皮的水提取物（AE）和乙醇提取物（EE）对利血平化小鼠（2至3个月大的雌性和雄性瑞士白化小鼠）的影响。通过连续3天（第1至3天）注射利血平（1毫克/千克/天，腹腔注射）诱导二元组状态。然后，在第4至8天用植物提取物（25、50和100毫克/千克/天，腹腔注射）或L-色氨酸（100毫克/千克/天，腹腔注射）对动物进行治疗。在第4天和第8天评估疼痛样（触觉和冷痛觉过敏）和抑郁样（悬尾、强迫游泳试验）行为参数。在第9天，处死动物以定量脑或脊髓中的乙酰胆碱酯酶活性、氧化应激参数、总儿茶酚胺、多巴胺、血清素、白细胞介素-1β和肿瘤坏死因子-α水平。还测定了血清中的白细胞介素-1β和肿瘤坏死因子-α。对EE进行了急性毒性和植物化学分析。AE和EE均可逆转利血平诱导的触觉和冷痛觉过敏、抑郁样行为、血清白细胞介素-1β和肿瘤坏死因子-α增加、脑乙酰胆碱酯酶活性以及儿茶酚胺浓度降低。植物提取物显著增加了脑和/或脊髓中的多巴胺水平并降低了氧化应激。对脑血清素和肿瘤坏死因子-α未观察到显著影响。EE表现出最佳的药理活性且无毒。液相色谱-串联质谱分子网络植物化学分析确定了5种高度确定的化合物，包括胡椒碱、醋酸橙酰胺和曲霉酚酸。AE和EE对疼痛和抑郁有效。它们的药理活性可能与炎症、氧化应激和儿茶酚胺的调节以及生物活性天然产物的存在有关。
