Duque Cristiane, Rabelo Rafaela Laruzo, Braga Gabriela Pacheco de Almeida, Mendes Soares Igor Paulino, de Souza Maria Eduarda, Chrisostomo Daniela Alvim, Hebling Josimeri, de Souza Costa Carlos Alberto, Prakki Anuradha, Regasini Luís Octavio
Universidade Católica Portuguesa (UCP), Faculty of Dental Medicine, Center for Interdisciplinary Research in Health (CIIS), Estrada da Circunvalação, s/n 3504-505, Viseu, Portugal.
São Paulo State University (UNESP), Department of Preventive and Restorative Dentistry, Araçatuba Dental School, R. José Bonifácio, 1193, Araçatuba 16015-050, Brazil.
Scientifica (Cairo). 2025 Feb 28;2025:3233536. doi: 10.1155/sci5/3233536. eCollection 2025.
Odontoblasts are cells specialized in dentin matrix deposition and the first line of defense when the dentin-pulp complex is injured by pathological processes, such as dental caries and trauma. Natural compounds, such as flavonoids, could be useful to stimulate odontoblast activity and reparative dentinogenesis in vital pulp therapies, especially in immature permanent teeth. This study evaluated the effect of flavonoids on odontoblast secretory activity and the expression of dentinogenesis markers. The effect of flavonoids was evaluated on phenotypic mineralization markers (alkaline phosphatase (ALP) activity and mineralized nodule deposition) by colorimetric assays and on the expression of , , , , and genes in odontoblast-like cells by quantitative polymerase chain reaction. Most of the flavonoids did not show toxicity between 100 and 25 μM. In distinct concentrations, epigallocatechin gallate (EGCG), taxifolin, myricetin, quercetin, and kaempferol stimulated the activity of ALP and increased mineralized nodule deposition. However, the highest effect on those phenotypic markers was observed after EGCG and taxifolin treatments. Then, they were selected for evaluation of gene expression. mRNA levels of and highly increased with taxifolin treatment, and expression was increased for both taxifolin and EGCG groups, without difference between them. and expression was not affected by these flavonoids. In conclusion, EGCG and taxifolin positively affect phenotypic mineralization markers; in particular, taxifolin highly stimulates early- and late-stage dentinogenesis genes.
成牙本质细胞是专门负责牙本质基质沉积的细胞，当牙本质 - 牙髓复合体因诸如龋齿和创伤等病理过程而受损时，它们是第一道防线。天然化合物，如黄酮类化合物，在活髓治疗中，特别是在未成熟恒牙中，可能有助于刺激成牙本质细胞活性和修复性牙本质形成。本研究评估了黄酮类化合物对成牙本质细胞分泌活性和牙本质形成标志物表达的影响。通过比色法评估黄酮类化合物对表型矿化标志物（碱性磷酸酶（ALP）活性和矿化结节沉积）的影响，并通过定量聚合酶链反应评估黄酮类化合物对成牙本质样细胞中、、、和基因表达的影响。大多数黄酮类化合物在100至25μM之间未显示出毒性。在不同浓度下，表没食子儿茶素没食子酸酯（EGCG）、紫杉叶素、杨梅素、槲皮素和山奈酚刺激了ALP的活性并增加了矿化结节沉积。然而，在EGCG和紫杉叶素处理后，观察到对这些表型标志物的影响最大。然后，选择它们进行基因表达评估。紫杉叶素处理后和的mRNA水平显著升高，紫杉叶素和EGCG组的表达均增加，两者之间无差异。和的表达不受这些黄酮类化合物的影响。总之，EGCG和紫杉叶素对表型矿化标志物有积极影响；特别是，紫杉叶素高度刺激早期和晚期牙本质形成基因。
