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掺入聚N-乙烯基己内酰胺水凝胶中的黄酮类化合物的细胞毒性和生物矿化潜力

Cytotoxicity and Biomineralization Potential of Flavonoids Incorporated into PNVCL Hydrogels.

作者信息

Braga Gabriela Pacheco de Almeida, Caiaffa Karina Sampaio, Rabelo Rafaela Laruzo, Santos Vanessa Rodrigues Dos, Souza Amanda Caselato Andolfatto, Ribeiro Lucas da Silva, Camargo Emerson Rodrigues de, Prakki Anuradha, Duque Cristiane

机构信息

Department of Preventive and Restorative Dentistry, Araçatuba Dental School, São Paulo State University (UNESP), Araçatuba 16015-050, SP, Brazil.

Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos 13565-905, SP, Brazil.

出版信息

J Funct Biomater. 2023 Mar 2;14(3):139. doi: 10.3390/jfb14030139.

DOI:10.3390/jfb14030139
PMID:36976063
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10058549/
Abstract

This study aimed to evaluate the effects of flavonoids incorporated into poly(N-vinylcaprolactam) (PNVCL) hydrogel on cell viability and mineralization markers of odontoblast-like cells. MDPC-23 cells were exposed to ampelopsin (AMP), isoquercitrin (ISO), rutin (RUT) and control calcium hydroxide (CH) for evaluation of cell viability, total protein (TP) production, alkaline phosphatase (ALP) activity and mineralized nodule deposition by colorimetric assays. Based on an initial screening, AMP and CH were loaded into PNVCL hydrogels and had their cytotoxicity and effect on mineralization markers determined. Cell viability was above 70% when MDPC-23 cells were treated with AMP, ISO and RUT. AMP showed the highest ALP activity and mineralized nodule deposition. Extracts of PNVCL+AMP and PNVCL+CH in culture medium (at the dilutions of 1/16 and 1/32) did not affect cell viability and stimulated ALP activity and mineralized nodules' deposition, which were statistically higher than the control in osteogenic medium. In conclusion, AMP and AMP-loaded PNVCL hydrogels were cytocompatible and able to induce bio-mineralization markers in odontoblast-cells.

摘要

本研究旨在评估掺入聚（N-乙烯基己内酰胺）（PNVCL）水凝胶中的类黄酮对成牙本质样细胞的细胞活力和矿化标志物的影响。将MDPC-23细胞暴露于蛇葡萄素（AMP）、异槲皮苷（ISO）、芦丁（RUT）和对照氢氧化钙（CH）中，通过比色法评估细胞活力、总蛋白（TP）产生、碱性磷酸酶（ALP）活性和矿化结节沉积。基于初步筛选，将AMP和CH加载到PNVCL水凝胶中，并测定其细胞毒性和对矿化标志物的影响。当MDPC-23细胞用AMP、ISO和RUT处理时，细胞活力高于70%。AMP显示出最高的ALP活性和矿化结节沉积。培养基中PNVCL+AMP和PNVCL+CH的提取物（稀释度为1/16和1/32）不影响细胞活力，并刺激ALP活性和矿化结节沉积，在成骨培养基中，其在统计学上高于对照组。总之，AMP和负载AMP的PNVCL水凝胶具有细胞相容性，能够诱导成牙本质细胞中的生物矿化标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2861/10058549/5773603c37fd/jfb-14-00139-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2861/10058549/5773603c37fd/jfb-14-00139-g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2861/10058549/5773603c37fd/jfb-14-00139-g009.jpg

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