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通过冻干法制备的miR-34a功能化羟基磷灰石促进了辐射性骨缺损的骨再生。

MiR-34a-Functionalized Hydroxyapatite by Lyophilization Promoted Bone Regeneration in Irradiated Bone Defects.

作者信息

Wu Xi, Feng Xiaoke, Zhang Gang, Liu Huan

机构信息

Department of Stomatology, Xinqiao Hospital, Army Medical University, Chongqing 400037, China.

Department of Prosthodontics, Tianjin Stomatological Hospital, School of Medicine, Nankai University, Tianjin 300041, China.

出版信息

J Tissue Eng Regen Med. 2023 Sep 11;2023:9946012. doi: 10.1155/2023/9946012. eCollection 2023.

DOI:10.1155/2023/9946012
PMID:40226426
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11918688/
Abstract

The rehabilitation of bone defects after radiotherapy requires the development of osteoinductive bone substitutes. MicroRNA could be used as an osteogenic factor to fabricate functional materials for bone regeneration. In this study, we used miR-34a to enhance bone regeneration after irradiation. We lyophilized lipofectamine-agomiR-34a lipoplexes on hydroxyapatite (HA) to develop miR-34a-functionalized hydroxyapatite (HA-agomiR-34a). The morphology was observed by scanning electron microscope and atomic force microscope. Fluorescence microscopy confirmed the retention of agomiR-34a on the surface of HA. HA-agomiR-34a showed high transfection efficiency and good biocompatibility. HA-agomiR-34a enhanced the osteoblastic differentiation of radiation-impaired bone marrow stromal cells (BMSCs). Implantation of HA-agomiR-34a promoted bone regeneration in irradiated bone defects. HA-agomiR-34a may be a novel and safe bone substitute to promote the reconstruction of bone defects after radiotherapy.

摘要

放疗后骨缺损的修复需要开发具有骨诱导性的骨替代物。微小RNA可用作成骨因子来制备用于骨再生的功能材料。在本研究中，我们使用miR-34a来促进放疗后的骨再生。我们将脂质体-反义miR-34a脂质复合物冻干在羟基磷灰石（HA）上，以制备miR-34a功能化的羟基磷灰石（HA-反义miR-34a）。通过扫描电子显微镜和原子力显微镜观察其形态。荧光显微镜证实了反义miR-34a保留在HA表面。HA-反义miR-34a表现出高转染效率和良好的生物相容性。HA-反义miR-34a增强了受辐射损伤的骨髓间充质干细胞（BMSC）的成骨分化。植入HA-反义miR-34a可促进放疗后骨缺损处的骨再生。HA-反义miR-34a可能是一种新型且安全的骨替代物，可促进放疗后骨缺损的修复。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/68f92424e712/JTERM2023-9946012.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/d33564f8d4b3/JTERM2023-9946012.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/497b21d91706/JTERM2023-9946012.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/67101247f73d/JTERM2023-9946012.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/108c6bb7b675/JTERM2023-9946012.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/1a6934654dcc/JTERM2023-9946012.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/b294e2cbb519/JTERM2023-9946012.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/e4c02c1205a8/JTERM2023-9946012.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/68f92424e712/JTERM2023-9946012.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/d33564f8d4b3/JTERM2023-9946012.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/497b21d91706/JTERM2023-9946012.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/67101247f73d/JTERM2023-9946012.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/108c6bb7b675/JTERM2023-9946012.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/1a6934654dcc/JTERM2023-9946012.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/b294e2cbb519/JTERM2023-9946012.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/e4c02c1205a8/JTERM2023-9946012.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ffd/11918688/68f92424e712/JTERM2023-9946012.008.jpg

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本文引用的文献

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Front Bioeng Biotechnol. 2022 Sep 19;10:952670. doi: 10.3389/fbioe.2022.952670. eCollection 2022.
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Local delivery and controlled release of miR-34a loaded in hydroxyapatite/mesoporous organosilica nanoparticles composite-coated implant wire to accelerate bone fracture healing.
载 miR-34a 的羟基磷灰石/介孔有机硅纳米复合涂层植入丝的局部递送和控释，以加速骨骨折愈合。
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Engineering precision nanoparticles for drug delivery.工程化精准纳米颗粒用于药物递送。
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Biomimetic Synthesis of Nanocrystalline Hydroxyapatite Composites: Therapeutic Potential and Effects on Bone Regeneration.仿生合成纳米晶羟基磷灰石复合材料：治疗潜力及其对骨再生的影响。
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