Jiang Kaiyun, Li Zhong, Zeng Xiangyu, Chen Xiangsheng, Liang Shuang, Zhang Wensong
Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang, 550025 China Guizhou University Guiyang China.
MycoKeys. 2025 Apr 3;115:363-381. doi: 10.3897/mycokeys.115.144522. eCollection 2025.
(Sieb. et Zucc.) Maxim, a perennial herb belonging to the Berberidaceae family, is widely used in traditional Chinese medicine for its beneficial role in enhancing kidney function, strengthening bones and muscles, and dispelling wind-dampness. Clinically, it is commonly used to treat osteoporosis, rheumatism, hypertension, and cardiovascular diseases. During 2023 to 2024, a disease suspected to be anthracnose was observed to be infecting the bases of seedlings in Bibo Town, Kaili City, Guizhou Province. In the fall, the disease incidence reached 90%, with severe infection resulting in total desiccation and foliage death. Tissue isolation and single-conidium methods were used to identify and isolate the pathogens, which were determined to be two anthracnose strains. Multi-locus phylogenetic analysis using ITS, , , , , , and , and morphological observations of representative isolates indicated that the two isolated fungal strains were new species belonging to the genus , namely and . Pathogenicity tests, adhering to Koch's postulates, confirmed that both fungi could infect ; exhibited a higher pathogenicity than . The present study provides valuable information regarding the prevention of anthracnose.
（西伯. 及祖克.）马克西姆，一种属于小檗科的多年生草本植物，在传统中药中被广泛使用，因其在增强肾功能、强健骨骼和肌肉以及祛风除湿方面具有有益作用。临床上，它常用于治疗骨质疏松症、风湿病、高血压和心血管疾病。在2023年至2024年期间，在贵州省凯里市碧波镇观察到一种疑似炭疽病的病害正在感染幼苗基部。秋季时，发病率达到90%，严重感染导致完全干枯和叶片死亡。采用组织分离和单孢分离法对病原菌进行鉴定和分离，确定为两种炭疽病菌株。利用ITS、、、、、、和进行多位点系统发育分析以及对代表性分离株的形态学观察表明，这两种分离的真菌菌株是属于属的新物种，即和。致病性试验遵循科赫法则，证实这两种真菌都能感染；表现出比更高的致病性。本研究为预防炭疽病提供了有价值的信息。
