Two new species of (Glomerellales, Glomerellaceae) causing anthracnose on .

(Sieb. et Zucc.) Maxim, a perennial herb belonging to the Berberidaceae family, is widely used in traditional Chinese medicine for its beneficial role in enhancing kidney function, strengthening bones and muscles, and dispelling wind-dampness. Clinically, it is commonly used to treat osteoporosis, rheumatism, hypertension, and cardiovascular diseases. During 2023 to 2024, a disease suspected to be anthracnose was observed to be infecting the bases of seedlings in Bibo Town, Kaili City, Guizhou Province. In the fall, the disease incidence reached 90%, with severe infection resulting in total desiccation and foliage death. Tissue isolation and single-conidium methods were used to identify and isolate the pathogens, which were determined to be two anthracnose strains. Multi-locus phylogenetic analysis using ITS, , , , , , and , and morphological observations of representative isolates indicated that the two isolated fungal strains were new species belonging to the genus , namely and . Pathogenicity tests, adhering to Koch's postulates, confirmed that both fungi could infect ; exhibited a higher pathogenicity than . The present study provides valuable information regarding the prevention of anthracnose.