Varas Condori Miguel Angel, Arias-Santé María Fernanda, Bridi Raquel, Rincón-Cervera Miguel Ángel, Porras Omar, Reyes-Jara Angélica, de Camargo Adriano Costa
Nutrition and Food Technology Institute, University of Chile, Santiago 7830490, Chile.
Departamento de Química Farmacológica y Toxicológica, Facultad de Ciencias Químicas y Farmacéuticas, Universidad de Chile, Santiago 8380000, Chile.
Antioxidants (Basel). 2025 Feb 26;14(3):263. doi: 10.3390/antiox14030263.
Growing concern about food quality and safety has driven the search for natural food additives. Furthermore, maqui leaf residue (MLR), recovered from infusions that have long been part of traditional Chilean herbal medicine, still conserves a high content of phenolic compounds. The aim of this study was to optimize the ultrasound-assisted extraction (UAE) of phenolics from MLR and evaluate their potential effect on salmon preservation. The optimized parameters for UAE (6 min, MLR:Water = 1:30, and 70 W), obtained by using a Box-Behnken design, rendered the highest total phenolic content and antioxidant capacity. Furthermore, UAE showed a higher concentration of phenolic compounds compared to conventional extraction techniques, as evaluated by UPLC-ESI-MS/MS. The salmon with MLR had up to four times lower levels of thiobarbituric acid reactive substances, induced by ozonated water treatment, than the sample without this natural antioxidant. Moreover, MLR exhibited similar or superior effectiveness compared to synthetic antioxidants, such as butylated hydroxyanisole (BHA), 3,5-Di-tert-4butylhydroxytoluene (BHT). This study highlights the use of short-time aqueous ultrasound extraction as an environmentally friendly technology that allows the recovery of phenolic compounds from MLR, with potential application as a natural preservative that may replace or decrease the use of BHA and/or BHT.
对食品质量和安全的日益关注推动了对天然食品添加剂的探索。此外，从长期以来一直是智利传统草药一部分的浸液中回收的马基叶残渣（MLR），仍保留着高含量的酚类化合物。本研究的目的是优化从MLR中超声辅助提取酚类物质的工艺，并评估其对三文鱼保鲜的潜在效果。通过Box-Behnken设计获得的超声辅助提取（UAE）优化参数（6分钟、MLR:水 = 1:30、70瓦），使总酚含量和抗氧化能力达到最高。此外，通过超高效液相色谱-电喷雾串联质谱（UPLC-ESI-MS/MS）评估，与传统提取技术相比，UAE显示出更高浓度的酚类化合物。经臭氧水处理诱导产生的硫代巴比妥酸反应性物质水平，含有MLR的三文鱼比不含这种天然抗氧化剂的样品低四倍之多。此外，与合成抗氧化剂如丁基羟基茴香醚（BHA）、3,5-二叔丁基-4-羟基甲苯（BHT）相比，MLR表现出相似或更优的效果。本研究强调了短时间水相超声提取作为一种环境友好型技术的应用，该技术能够从MLR中回收酚类化合物，具有作为天然防腐剂替代或减少BHA和/或BHT使用的潜在应用价值。
