de Camargo Adriano Costa, Concepción Alvarez Alina, Arias-Santé María Fernanda, Oyarzún Juan Esteban, Andia Marcelo E, Uribe Sergio, Núñez Pizarro Paula, Bustos Simón M, Schwember Andrés R, Shahidi Fereidoon, Bridi Raquel
Nutrition and Food Technology Institute, University of Chile, Santiago 7830490, Chile.
Biomedical Imaging Center, School of Medicine, Pontificia Universidad Católica de Chile, ANID-Millennium Institute for Intelligent Healthcare Engineering-iHEALTH, Santiago 7820436, Chile.
Antioxidants (Basel). 2022 Jun 10;11(6):1139. doi: 10.3390/antiox11061139.
Chickpeas are rich sources of bioactive compounds such as phenolic acids, flavonoids, and isoflavonoids. However, the contribution of insoluble-bound phenolics to their antioxidant properties remains unclear. Four varieties of chickpeas were evaluated for the presence of soluble (free and esterified) and insoluble-bound phenolics as well as their antiradical activity, reducing power and inhibition of peroxyl-induced cytotoxicity in human HuH-7 cells. In general, the insoluble-bound fraction showed a higher total phenolic content. Phenolic acids, flavonoids, and isoflavonoids were identified and quantified by UPLC-MS/MS. Taxifolin was identified for the first time in chickpeas. However, -hydroxybenzoic acid, taxifolin, and biochanin A were the main phenolics found. Biochanin A was mostly found in the free fraction, while -hydroxybenzoic acid was present mainly in the insoluble-bound form. The insoluble-bound fraction made a significant contribution to the reducing power and antiradical activity towards peroxyl radical. Furthermore, all extracts decreased the oxidative damage of human HuH-7 cells induced by peroxyl radicals, thus indicating their hepatoprotective potential. This study demonstrates that the antioxidant properties and bioactive potential of insoluble-bound phenolics of chickpeas should not be neglected.
鹰嘴豆是生物活性化合物的丰富来源，如酚酸、黄酮类化合物和异黄酮。然而，不溶性结合酚类对其抗氧化特性的贡献仍不清楚。对四个鹰嘴豆品种进行了评估，以检测可溶性（游离和酯化）和不溶性结合酚类的存在情况，以及它们的抗自由基活性、还原能力和对人HuH-7细胞中过氧自由基诱导的细胞毒性的抑制作用。总体而言，不溶性结合部分显示出更高的总酚含量。通过超高效液相色谱-串联质谱法（UPLC-MS/MS）对酚酸、黄酮类化合物和异黄酮进行了鉴定和定量。在鹰嘴豆中首次鉴定出了 taxifolin。然而，对羟基苯甲酸、taxifolin 和鹰嘴豆芽素A是主要发现的酚类物质。鹰嘴豆芽素A主要存在于游离部分，而对羟基苯甲酸主要以不溶性结合形式存在。不溶性结合部分对过氧自由基的还原能力和抗自由基活性有显著贡献。此外，所有提取物都降低了过氧自由基诱导的人HuH-7细胞的氧化损伤，从而表明它们具有肝脏保护潜力。这项研究表明，鹰嘴豆不溶性结合酚类的抗氧化特性和生物活性潜力不应被忽视。
