Zhang Leimin, Huang Dongyu, Gu Jiaze, Liang Hualiang, Ren Mingchun
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Antioxidants (Basel). 2025 Mar 5;14(3):313. doi: 10.3390/antiox14030313.
A 12-week aquaculture trial was conducted to evaluate the effects of vitamin B on the intestinal health of largemouth bass (). Six feeds with a vitamin B content of 2.03 (control group), 2.91, 3.30, 6.03, 9.53, and 21.79 mg/kg were prepared. The results were as follows. Regarding digestive efficiency, the 9.53 mg/kg group showed significantly higher activities of AMY, LPS, and TRY compared to the control group; the 6.03 mg/kg group exhibited increased AKP and Na/K ATPase activities. Regarding immunity, the 6.03 mg/kg group had markedly higher relative expressions of and than the control group; the 9.53 mg/kg group showed significantly higher relative expressions of , , , and , while and were notably lower, and was noticeably decreased in 21.79 mg/kg group. For antioxidant capacity, the 6.03 mg/kg group had markedly higher activities of CAT, SOD, GSH-Px, and T-AOC levels, compared to the control group; the MDA level in the control group was markedly higher than in the other groups. The relative expressions of , , , and were highest in 9.53 mg/kg group and significantly higher than in the control group. In conclusion, an appropriate level of vitamin B in the feed is vital for protecting the intestinal health of largemouth bass.
进行了一项为期12周的水产养殖试验，以评估维生素B对大口黑鲈肠道健康的影响。制备了六种维生素B含量分别为2.03（对照组）、2.91、3.30、6.03、9.53和21.79 mg/kg的饲料。结果如下。在消化效率方面，9.53 mg/kg组的淀粉酶（AMY）、脂肪酶（LPS）和胰蛋白酶（TRY）活性显著高于对照组；6.03 mg/kg组的碱性磷酸酶（AKP）和钠钾ATP酶（Na/K ATPase）活性有所增加。在免疫力方面，6.03 mg/kg组的[具体基因1]和[具体基因2]相对表达量明显高于对照组；9.53 mg/kg组的[具体基因3]、[具体基因4]、[具体基因5]和[具体基因6]相对表达量显著更高，而[具体基因7]和[具体基因8]明显更低，21.79 mg/kg组的[具体基因9]明显降低。在抗氧化能力方面，6.03 mg/kg组的过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性和总抗氧化能力（T-AOC）水平明显高于对照组；对照组的丙二醛（MDA）水平明显高于其他组。9.53 mg/kg组的[具体基因10]、[具体基因11]、[具体基因12]和[具体基因13]相对表达量最高，且显著高于对照组。总之，饲料中适当水平的维生素B对保护大口黑鲈的肠道健康至关重要。
