Castro Carolina, Coutinho Filipe, Iglesias Paula, Oliva-Teles Aires, Couto Ana
Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR/CIMAR), Terminal de Cruzeiros do Porto de Leixões, Matosinhos, Portugal.
Buggypower, Parque Industrial Base 2000, Lorquí-Murcia, Spain.
Front Vet Sci. 2020 Dec 17;7:607575. doi: 10.3389/fvets.2020.607575. eCollection 2020.
This study aimed to evaluate the effects of including microalgae sp. or sp. in plant-based diets on antioxidant mechanisms of European sea bass () juveniles. For this purpose, three isoproteic (50%) and isolipidic (19%) diets were formulated: a practical diet, containing 15% fish meal (FM) and plant ingredients as the protein source and a mixture of fish oil and vegetable oils (40: 60) as lipid source (control diet); and two diets identical to the control but with the FM replaced by sp. or sp. (diets Nanno and Chlo, respectively). The diets were offered to quadruplicate groups of 25 fish (initial body weight: 24 ± 1 g) for 11 weeks and then enzymatic and non-enzymatic antioxidant mechanisms and lipid oxidative biomarkers were assessed in the liver and intestine of these fish. Results showed that the antioxidant response was tissue-dependent, with the liver exhibiting lower glutathione peroxidase and glucose-6-phosphate dehydrogenase (only in Chlo group) activities, and intestine lower superoxide dismutase activity with the diets including microalgae compared to control diet. An increase of oxidized glutathione content was also observed in the intestine of fish fed the microalgae diets. Catalase and glutathione reductase activities, oxidative stress index, and total and reduced glutathione, were unaffected by dietary treatments in both tissues. Overall, the lipid peroxidation status was not compromised by the replacement of FM by microalgae.
本研究旨在评估在欧洲鲈鱼幼鱼的植物性日粮中添加微藻物种对其抗氧化机制的影响。为此，配制了三种等蛋白（50%）和等脂（19%）的日粮：一种实用日粮，含有15%的鱼粉（FM）和植物成分作为蛋白质来源，以及鱼油和植物油的混合物（40:60）作为脂质来源（对照日粮）；另外两种日粮与对照日粮相同，但分别用微藻物种或替代了FM（分别为Nanno日粮和Chlo日粮）。将这些日粮投喂给四组每组25尾鱼（初始体重：24±1克），持续11周，然后评估这些鱼肝脏和肠道中的酶促和非酶促抗氧化机制以及脂质氧化生物标志物。结果表明，抗氧化反应具有组织依赖性，与对照日粮相比，在含微藻的日粮中，肝脏中的谷胱甘肽过氧化物酶和葡萄糖-6-磷酸脱氢酶（仅在Chlo组）活性较低，肠道中的超氧化物歧化酶活性较低。在喂食微藻日粮的鱼的肠道中还观察到氧化型谷胱甘肽含量增加。过氧化氢酶和谷胱甘肽还原酶活性、氧化应激指数以及总谷胱甘肽和还原型谷胱甘肽在两个组织中均不受日粮处理的影响。总体而言，微藻替代FM并未损害脂质过氧化状态。
