Ma Shuangshuang, Kang Lingtao, Gao Zhipeng, Pan Zhaoping, Huang Lvhong, Chen Jiaxu, Liao Yanfang, Guo Jiajing, Fu Fuhua
Longping Branch, College of Biology, Hunan University, Changsha 410125, China.
Dongting Laboratory, Hunan Agricultural Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
Antioxidants (Basel). 2025 Mar 14;14(3):343. doi: 10.3390/antiox14030343.
Chronic and excessive alcohol consumption induces alcohol-related liver injury (ALI), characterized by oxidative stress (OS), disrupted lipid metabolism, and gut microbiota dysbiosis. Given the lack of effective pharmacological treatments, flavonoid-rich fruits have attracted growing attention as potential intervention strategies. This study investigated the independent and combined effects of extracts from pericarpium (CRPE) and (Sweet) Nakai (CSPE), previously shown to possess hepatoprotective properties, in a mouse model of ethanol-induced chronic ALI. The flavonoid composition of CRPE and CSPE was characterized using LC-MS/MS, and their potential mechanisms of action were further elucidated through transcriptomic analysis. The results showed that CRPE and CSPE, whether administered individually or in combination, effectively alleviated alcohol-induced hepatic histological damage and inflammatory responses. Furthermore, both extracts significantly reduced OS and improved lipid metabolism. Notably, CRPE, CSPE, and their combination regulated the gut microbiota, as shown by increased abundances of beneficial bacteria such as and , along with elevated levels of short-chain fatty acids (SCFAs). These findings highlight that combinations of multiple fruit extracts exhibit significant potential in alleviating ALI by modulating the gut microbiota, providing valuable insights for the development of functional foods.
长期过量饮酒会导致酒精性肝损伤（ALI），其特征为氧化应激（OS）、脂质代谢紊乱和肠道微生物群失调。鉴于缺乏有效的药物治疗方法，富含类黄酮的水果作为潜在的干预策略受到越来越多的关注。本研究在乙醇诱导的慢性ALI小鼠模型中，研究了先前已证明具有肝脏保护特性的枳椇子果皮提取物（CRPE）和北枳椇提取物（CSPE）的独立作用及联合作用。使用液相色谱-串联质谱法（LC-MS/MS）对CRPE和CSPE的类黄酮成分进行了表征，并通过转录组分析进一步阐明了它们潜在的作用机制。结果表明，CRPE和CSPE无论是单独给药还是联合给药，均能有效减轻酒精诱导的肝脏组织学损伤和炎症反应。此外，两种提取物均显著降低了氧化应激并改善了脂质代谢。值得注意的是，CRPE、CSPE及其组合调节了肠道微生物群，表现为双歧杆菌和嗜酸乳杆菌等有益细菌的丰度增加，以及短链脂肪酸（SCFAs）水平升高。这些发现突出表明，多种水果提取物的组合在通过调节肠道微生物群减轻ALI方面具有显著潜力，为功能性食品的开发提供了有价值的见解。
