Leffak M, Trempe J P
Nucleic Acids Res. 1985 Jul 11;13(13):4853-69. doi: 10.1093/nar/13.13.4853.
We have studied the assembly of histone H1 and the high mobility group nonhistones 14/17 by isopycnic analysis after crosslinking density labeled MSB cell nuclei or chromatin. Carbodiimide crosslinking produces dense poly-H1 and hybrid density H1-H2A histone dimers, indicating that new H1 is deposited nonrandomly, albeit nonconservatively relative to new core histones. Core histone-HMG crosslinking with succinimidyl propionate yields dense HMG 14 in uniformly dense particles and new HMG 17 crosslinked to both dense and light protein, implying that HMG 14 and 17 each deposit nonrandomly; but differently with respect to new core octamers. Propionimidate crosslinking yields dense H1-HMG 17 dimers, suggesting that the interactions of new 14/17 with H1 (new HMG 14-old H1, new HMG 17-new H1) are reciprocal to their interactions with the core histones.
我们通过对密度标记的MSB细胞核或染色质进行交联后的等密度分析，研究了组蛋白H1和高迁移率族非组蛋白14/17的组装情况。碳二亚胺交联产生致密的多聚H1和混合密度的H1-H2A组蛋白二聚体，这表明新的H1是非随机沉积的，尽管相对于新的核心组蛋白而言是非保守性沉积。用琥珀酰亚胺丙酸酯进行核心组蛋白-HMG交联，在均匀致密的颗粒中产生致密的HMG 14，新的HMG 17与致密和轻质蛋白都发生交联，这意味着HMG 14和17各自都是非随机沉积的；但相对于新的核心八聚体而言有所不同。丙基亚氨酸酯交联产生致密的H1-HMG 17二聚体，这表明新的14/17与H1之间的相互作用（新的HMG 14-旧的H1，新的HMG 17-新的H1）与其与核心组蛋白的相互作用是相互的。
