核小体组装保守模式的稳定性。

Nucleic Acids Res. 1983 May 11;11(9):2717-32. doi: 10.1093/nar/11.9.2717.

The conservative assembly of nucleosome histone octamer cores has been confirmed by electrophoretic analysis of density labeled histones following equilibrium buoyant density centrifugation. After normal replication, crosslinked octamers are shown not to contain a mixture of new and old core histones. Moreover, when DNA synthesis is inhibited by ara-C nucleosome cores are still assembled exclusively from nascent histone. Similarly, after release from cycloheximide inhibition newly synthesized core histone is conservatively deposited. Thus, a conservative mechanism of histone octamer assembly occurs when nascent histone is present in the normal stoichiometry to nascent DNA and when chromatin is assembled in nascent histone or nascent DNA excess.

通过平衡浮力密度离心后对密度标记组蛋白的电泳分析，已证实核小体组蛋白八聚体核心的保守组装。正常复制后，交联的八聚体不含新老核心组蛋白的混合物。此外，当DNA合成被阿糖胞苷抑制时，核小体核心仍仅由新生组蛋白组装而成。同样，从环己酰亚胺抑制中释放后，新合成的核心组蛋白会保守沉积。因此，当新生组蛋白与新生DNA以正常化学计量存在，且染色质在新生组蛋白或新生DNA过量的情况下组装时，会发生组蛋白八聚体组装的保守机制。