Akhtach Sihame, Tabia Zakaria, Belkhou Rajae, Mabrouk Khalil El
Laboratory of Biotechnology, Environment, Agrifood, and Health (LBEAS) Faculty of Science Dhar Mahraz, University Sidi Mohamed Ben Abdallah, Fes, Morocco.
Euromed University of Fes, Fes, Morocco.
Int J Pharm. 2025 May 15;676:125594. doi: 10.1016/j.ijpharm.2025.125594. Epub 2025 Apr 12.
Healing of chronic wounds requires interactive dressings that not only meet basic biological criteria, of biocompatibility, but also offer additional functionalities such as antioxidant and antibacterial properties. In this study, three novel multifunctional nanofibrous membranes based on EPS, an α-glucan exopolysaccharide produced by Lacticaseibacillus rhamnosus P14, blended with PEO as a co-polymer were successfully developed using electrospinning. The membranes incorporated Cu or Ag-doped bioactive glass (BG) nanoparticles, to enhance their functionalities. The structural and thermal properties of the electrospun membranes were characterized using FT-IR, TGA, and DSC analysis. In addition, the surface morphology, fiber size, and porosity were examined by SEM analysis. Additionally, their biological properties, including antioxidant and antibacterial activities, were thoroughly investigated. SEM confirmed the effective electrospinning of the EPS-PEO and EPS-PEO-BG blends from aqueous solutions using optimized process parameters, resulting in the successful incorporation of the BG nanoparticles and uniform fibers with average diameter ranging from 270 to 352 nm. Moreover, DPPH RSA and FRAP assays showed a significant antioxidant capacity for all prepared membranes which is attributed to the EPS component. Moreover, the antibacterial activity revealed a notable inhibition against E. coli and S. aureus after 24 h exposure to the composite membranes. This work presents a novel synergistic approach to developing multifunctional wound dressing by combining the antioxidant properties of EPS, the antibacterial activity of ion-doped bioactive glass nanoparticles, and structural benefits of electrospinning.
慢性伤口的愈合需要交互式敷料，这种敷料不仅要满足生物相容性等基本生物学标准，还要具备抗氧化和抗菌等附加功能。在本研究中，通过静电纺丝成功制备了三种新型多功能纳米纤维膜，它们基于鼠李糖乳杆菌P14产生的α-葡聚糖胞外多糖EPS，并与作为共聚物的PEO共混。这些膜掺入了铜或银掺杂的生物活性玻璃（BG）纳米颗粒，以增强其功能。采用傅里叶变换红外光谱（FT-IR）、热重分析（TGA）和差示扫描量热法（DSC）对静电纺丝膜的结构和热性能进行了表征。此外，通过扫描电子显微镜（SEM）分析研究了其表面形态、纤维尺寸和孔隙率。另外，还对它们的生物学特性，包括抗氧化和抗菌活性进行了深入研究。SEM证实，使用优化的工艺参数从水溶液中有效地静电纺制了EPS-PEO和EPS-PEO-BG共混物，成功地掺入了BG纳米颗粒，并形成了平均直径为270至352nm的均匀纤维。此外，二苯基苦味酰基自由基（DPPH）还原能力测定法和铁离子还原抗氧化能力（FRAP）测定法表明，所有制备的膜都具有显著的抗氧化能力，这归因于EPS成分。此外，抗菌活性显示，复合膜在暴露24小时后对大肠杆菌和金黄色葡萄球菌有显著抑制作用。这项工作提出了一种新颖的协同方法，通过结合EPS的抗氧化特性、离子掺杂生物活性玻璃纳米颗粒的抗菌活性以及静电纺丝的结构优势来开发多功能伤口敷料。
