Incorporation of 3H-labeled nucleosides and 3H-labeled deoxynucleosides into detergent soluble DNA.

Detergent soluble DNA from splenocytes of immunologically stimulated mice has been shown to incorporate [3H]dThd more rapidly than detergent insoluble DNA. In this report we compare the incorporation of other 3H-labeled nucleosides and 3H-labeled deoxynucleosides and the distribution of 3H in the different size classes of detergent soluble DNA. The order of incorporation into DS DNA is [3H]dThd greater than [3H]dCyd greater than [3H]Ado greater than [3H]dGuo approximately equal to [3H]Cyd greater than [3H]dAdo greater than [3H]Guo. We also show that the previously reported slight enrichment in Gua + Cyt content is not due to preferential incorporation of dGuo or of dCyd into any one size class.