Strauss P R, Banerjee P T, LaGree K, Mui S C
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7056-60. doi: 10.1073/pnas.81.22.7056.
We reported recently that splenocytes from concanavalin A-stimulated mice rapidly incorporated [3H]thymidine into non-mitochondrial DNA that was detergent soluble and distributed in size classes between 200 and 5000 base pairs. In this report we show that small [3H]thymidine-labeled oligonucleotides (less than 100 base pairs long) appear by 15 min. Subsequently, [3H]thymidine in small oligonucleotides diminishes as incorporation into larger size classes of detergent-soluble DNA occurs in a pattern that is stable for at least 3 hr. Although incorporation of [3H]thymidine into the oligonucleotides is not sensitive to aphidicolin or hydroxyurea, the appearance of [3H]thymidine in larger species is blocked by both drugs. These results indicate that the enzymatic process involved in synthesis of oligonucleotides is somehow distinct from the process involved in synthesis of larger detergent-soluble size classes. Synthesis of the latter may be replication related.
我们最近报道，来自伴刀豆球蛋白A刺激小鼠的脾细胞能迅速将[3H]胸苷掺入非线粒体DNA中，该DNA可溶于去污剂，大小在200至5000个碱基对之间。在本报告中，我们表明，15分钟时出现了小的[3H]胸苷标记的寡核苷酸（长度小于100个碱基对）。随后，随着[3H]胸苷掺入更大尺寸的可溶于去污剂的DNA中，小寡核苷酸中的[3H]胸苷减少，这种模式至少稳定3小时。虽然[3H]胸苷掺入寡核苷酸对阿非科林或羟基脲不敏感，但这两种药物均会阻断较大分子中[3H]胸苷的出现。这些结果表明，寡核苷酸合成所涉及的酶促过程在某种程度上不同于较大尺寸的可溶于去污剂的DNA合成所涉及的过程。后者的合成可能与复制有关。
