Lin Xiaomeng, Yang Hua, Cai Tingting, Yang Zhangshuo, Niu Siyun, Jia Hui
School of Clinical Medicine, Hebei University, Baoding, 071000, China.
Department of Breast Surgery, Affiliated Hospital of Hebei University, Baoding, 071000, China.
BMC Cancer. 2025 Apr 14;25(1):676. doi: 10.1186/s12885-025-13999-z.
The quality control testing component and main active ingredient of Polygonum multiflorum Thunb. (P. multiflorum), known as trans-2,3,5,4'-tetrahydroxystilbene 2-O-β-D-glucopyranoside (TSG), exhibits diverse biological activities. In this study, we report, for the first time, the potent ability of TSG to induce ferroptosis in triple negative breast cancer (TNBC) cell lines and to inhibit the proliferation of TNBC cells. Treatment with TSG triggers the production of lipid peroxides, 4-hydroxynonenal (4-HNE), and reactive oxygen species (ROS) in TNBC cells, indicating the induction of ferroptosis. Both in vivo and in vitro experiments confirmed the inhibitory effects of TSG on TNBC cell proliferation and metastasis. Furthermore, we investigated the effects of other stilbene glycoside oligomers, alongside TSG, on TNBC cell lines. These compounds also demonstrated the ability to induce ferroptosis and suppress TNBC cells' proliferation and metastasis. These findings suggest that the induction of ferroptosis by TSG and related compounds could potentially serve as a promising therapeutic strategy for TNBC treatment.
何首乌（Polygonum multiflorum Thunb.）的质量控制检测成分及主要活性成分，即反式-2,3,5,4'-四羟基二苯乙烯-2-O-β-D-吡喃葡萄糖苷（TSG），具有多种生物学活性。在本研究中，我们首次报道了TSG在三阴性乳腺癌（TNBC）细胞系中诱导铁死亡以及抑制TNBC细胞增殖的强大能力。用TSG处理会引发TNBC细胞中脂质过氧化物、4-羟基壬烯醛（4-HNE）和活性氧（ROS）的产生，表明诱导了铁死亡。体内和体外实验均证实了TSG对TNBC细胞增殖和转移的抑制作用。此外，我们研究了除TSG外的其他二苯乙烯苷低聚物对TNBC细胞系的影响。这些化合物也表现出诱导铁死亡以及抑制TNBC细胞增殖和转移的能力。这些发现表明，TSG及相关化合物诱导铁死亡可能是一种有前景的TNBC治疗策略。
