使用毛细管区带电泳对具有不同化学计量比的抗体-寡核苷酸缀合物进行无标记定量。

Label-free quantification of antibody-oligonucleotide conjugates with varying stoichiometries using capillary zone electrophoresis.

作者信息

Zhou Mo, Zhang Xiaoxia, Xia Qinglin, Liu Dongke, Chen Hongxu, Wen Yanli, Wang Lele, Guo Linjie, Li Jiang, Wang Lihua

机构信息

CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

University of Chinese Academy of Sciences, Beijing 100049, China.

出版信息

Analyst. 2025 May 12;150(10):2014-2018. doi: 10.1039/d5an00214a.

DOI:10.1039/d5an00214a

PMID:40232196

Abstract

We report a label-free capillary zone electrophoresis platform enabling quantitative analysis of antibody-oligonucleotide conjugate (AOC) heterogeneity with single-stranded oligonucleotide (21 nt) resolution. This methodology resolves free antibodies, oligonucleotides, and AOC subpopulations while precisely evaluating conjugation efficiency and oligonucleotide-to-antibody ratio (OAR) heterogeneity across three synthetic strategies.

摘要

我们报告了一种无标记毛细管区带电泳平台，该平台能够以单链寡核苷酸（21个核苷酸）分辨率对抗体 - 寡核苷酸缀合物（AOC）的异质性进行定量分析。这种方法能够分离游离抗体、寡核苷酸和AOC亚群，同时精确评估三种合成策略中的缀合效率和寡核苷酸与抗体比例（OAR）异质性。