Protective effects of Moringa oleifera leaf extract against cyclophosphamide-induced ovarian dysfunction and follicular loss in rats.

The current study aims to determine whether Moringa oleifera (M. oleifera) leaf extract can reverse cyclophosphamide (CP)-induced ovarian dysfunction and follicle loss in rats, potentially through antioxidant or anti-inflammatory pathways. Female rats were divided into four experimental groups: (1) negative control (administrated distilled water), (2) premature ovarian failure (POF) model group (induced by a single intraperitoneal dose of CP), (3) M. Oleifera extract alone, and (4) M. oleifera + CP. CP induced multiple effects on the ovaries, including hormonal imbalances (increased FSH and decreased E2 levels), oxidative stress (elevated serum MDA and NO levels), altered gene expression (upregulated TNF-α and downregulated TGF-β), and histological changes (follicular atresia and stromal hyperplasia). Pretreatment with M. oleifera successfully mitigated CP-induced oxidative and inflammatory changes, as well as ovarian tissue damage, but failed to reverse serum hormonal imbalances. These findings demonstrate the protective potential of M. oleifera leaf extract against CP-induced ovarian toxicity, likely mediated by the synergistic antioxidant, anti-inflammatory, and organ-protective properties of its bioactive components.