Hausenbauer J M, Waites W M, Setlow P
J Bacteriol. 1977 Feb;129(2):1148-50. doi: 10.1128/jb.129.2.1148-1150.1977.
As previously found for spores of Bacillus species, dormant spores of Clostridium bifermentans contained essentially no adenosine triphosphate, a high level of adenosine monophosphate, a high level of 3-phosphoglyceric acid, and much transfer ribonucleic acid lacking a 3'-terminal adenosine monophosphate residue. As in spores of Bacillus species, germination of C. bifermentans spores was accompanied by utilization of the 3-phosphoglyceric acid, a large increase in the adenosine triphosphate level, and the disappearance of defective transfer ribonucleic acid. In contrast to spores of Bacillus species, dormant spores of C. bifermentans contained little free amino acid.
正如之前在芽孢杆菌属的孢子中所发现的那样,双发酵梭菌的休眠孢子基本上不含三磷酸腺苷,含有高水平的一磷酸腺苷、高水平的3-磷酸甘油酸,以及大量缺少3'-末端一磷酸腺苷残基的转移核糖核酸。与芽孢杆菌属的孢子一样,双发酵梭菌孢子的萌发伴随着3-磷酸甘油酸的利用、三磷酸腺苷水平的大幅增加以及有缺陷的转移核糖核酸的消失。与芽孢杆菌属的孢子不同的是,双发酵梭菌的休眠孢子含有很少的游离氨基酸。