Wyatt L R, Waites W M
J Gen Microbiol. 1975 Aug;89(2):337-44. doi: 10.1099/00221287-89-2-337.
The effect of chlorine on the germination, outgrowth, colony formation and structure of spores of Clostridium bifermentans, Bacillus subtilis var. niger and Bacillus cereus was examined. Chlorine decreased heat resistance and slowed or prevented germination and swelling, but spores that did swell were usualy able to elongate to form vegetative cells. Chlorine removed protein from spores, apparently from the coat, and allowed lysozyme to initiate germination. Treatment with other agents that remove spore-coat protein increased the lethal effect of chlorine by as much as 4000-fold, suggesting that coat protein protects spores against chlorine.
研究了氯对双酶梭菌、枯草芽孢杆菌黑色变种和蜡样芽孢杆菌孢子的萌发、生长、菌落形成及结构的影响。氯降低了耐热性，减缓或阻止了萌发和肿胀，但已肿胀的孢子通常能够伸长形成营养细胞。氯从孢子中去除蛋白质，显然是从孢子衣中去除的，并使溶菌酶引发萌发。用其他去除孢子衣蛋白质的试剂处理，可使氯的致死效应增加多达4000倍，这表明孢子衣蛋白质可保护孢子免受氯的影响。
