Court J P, Bianco A E, Townson S, Ham P J, Friedheim E
Trop Med Parasitol. 1985 Jun;36(2):117-9.
The in vitro drug response of third stage larvae of the cattle filarial worm Onchocerca lienalis has been studied. Larvae were maintained in Minimum Essential Eagles medium supplemented with 10% inactivated foetal calf serum and exposed to the following drugs: Mel W, caparsolate sodium, suramin, ivermectin, flubendazole, levamisole and amoscanate plus 3 novel melaminylthioarsenites. Antiparasitic activity of these compounds has been assessed on their killing effects and their ability to totally inhibit the moult from the third to the fourth stage. Unfortunately, moulting occurred at a low rate in the culture system used and was not suitable as a quantitative parameter for describing drug activity. However, the results indicated that if the rate of ecdysis could be increased by improving the culture conditions, moulting could be used in this manner since exsheathment has inhibited by certain compounds at concentrations where killing effects were less than 50%. Of the compounds evaluated ivermectin demonstrated good activity, totally preventing ecdysis at 0.001 microgram/ml. The drug assay system used in this study may offer a starting point for the eventual development of a meaningful and specific in vitro screen for new anti-Onchocerca agents.
对牛丝状线虫链尾盘尾丝虫第三期幼虫的体外药物反应进行了研究。幼虫饲养于添加10%灭活胎牛血清的最低必需 Eagle 培养基中，并暴露于以下药物：Mel W、卡巴胂钠、苏拉明、伊维菌素、氟苯达唑、左旋咪唑和氨硫脲，以及3种新型三聚氰胺基硫代亚砷酸盐。已根据这些化合物的杀伤效果及其完全抑制从第三期到第四期蜕皮的能力评估了它们的抗寄生虫活性。遗憾的是，在所使用的培养系统中蜕皮发生率较低，不适宜作为描述药物活性的定量参数。然而，结果表明，如果通过改善培养条件提高蜕皮率，蜕皮可用于此目的，因为在杀伤效果小于50%的浓度下，某些化合物可抑制脱鞘。在所评估的化合物中，伊维菌素表现出良好的活性，在0.001微克/毫升时完全阻止蜕皮。本研究中使用的药物检测系统可能为最终开发有意义且特异的新型抗盘尾丝虫药物体外筛选方法提供一个起点。
