Pudney M, Litchfield T, Bianco A E, Mackenzie C D
Department of Medical Entomology, London School of Hygiene and Tropical Medicine, England.
Acta Trop. 1988 Mar;45(1):67-76.
Fourth-stage larvae (L4) of the cattle filarial parasite Onchocerca lienalis were produced from third stage forms (L3) and maintained in vitro using a variety of culture media and feeder cell layers. Up to 50% of the L3 larvae moulted to the fourth stage in the presence of two jird (Meriones unguiculatus) cell lines, a monkey kidney cell line and also with Vero cells. Moulting took place 2-5 days after of the initiation of the cultures: These L4 parasites could be maintained in a healthy condition for up to 88 days, although no significant increases in size of these larvae were observed during the culture period. The medium giving the most promising results, both in terms of moulting and survival, was 199 supplemented with heat inactivated foetal bovine serum (20%) and glucose (2 mg/ml). The feeder layer cells could be replaced by fresh jird red blood cells. Moulting of parasites occurred in medium alone but the viability was reduced under these conditions.
牛丝状寄生虫盘尾丝虫的第四期幼虫（L4）由第三期幼虫（L3）发育而来，并使用多种培养基和饲养细胞层在体外进行培养。在两种沙鼠（长爪沙鼠）细胞系、一种猴肾细胞系以及Vero细胞存在的情况下，高达50%的L3幼虫蜕变为第四期幼虫。蜕皮发生在培养开始后的2至5天：这些L4寄生虫可以在健康状态下维持长达88天，尽管在培养期间未观察到这些幼虫的大小有显著增加。在蜕皮和存活方面都给出最有前景结果的培养基是补充了热灭活胎牛血清（20%）和葡萄糖（2毫克/毫升）的199培养基。饲养层细胞可以用新鲜的沙鼠红细胞替代。寄生虫在单独的培养基中也会发生蜕皮，但在这些条件下其活力会降低。
