Wireman J W, Dworkin M
J Bacteriol. 1977 Feb;129(2):798-802. doi: 10.1128/jb.129.2.798-802.1977.
The developmental events during fruiting body construction by the myxobacterium M. xanthus is an orderly process characterized by several sequential stages: growth leads to aggregation leads to formation of raised, darkened mounds of cells leads to autolysis leads to myxospore induction. The temporal sequence of autolysis followed by myxospore induction is consistent with the interpretation that developmental autolysis provides essential requirements for the surviving cells to induce to myxospores. At intermediate developmental times on agar plates a fraction of the cell population is irreversibly committed to lyse; i.e., lysis continues in liquid growth medium or in magnesium-phosphate buffer. Lysis is cell concentration independent and is therefore likely to be by an autolytic mechanism. The lysis sequence can be preliminarily characterized as having an early stage during which deoxyribonucleic acid synthesis continues and a later irreversible stage during which deoxyribonucleic acid synthesis does not occur. Irreversible lysis in liquid growth medium or in magnesium-phosphate buffer is initiated on agar plates during nutrient deprivation and such lysis results in the induction of a fraction of the population to myxospores. This induction is dependent upon the concentration of lysis products, thus providing evidence that developmentally induced autolysis is required for myxospore induction.
黄色粘球菌在构建子实体过程中的发育事件是一个有序的过程，其特征在于几个连续的阶段：生长导致聚集，进而导致形成凸起、颜色变深的细胞堆，接着导致自溶，最终导致粘孢子诱导。自溶后紧接着粘孢子诱导的时间顺序与这样一种解释相一致，即发育性自溶为存活细胞诱导形成粘孢子提供了必要条件。在琼脂平板上发育的中间阶段，一部分细胞群体不可逆地注定要裂解；也就是说，在液体生长培养基或磷酸镁缓冲液中裂解仍会继续。裂解与细胞浓度无关，因此很可能是通过自溶机制进行的。裂解序列初步特征为具有一个早期阶段，在此阶段脱氧核糖核酸合成仍在继续，以及一个后期不可逆阶段，在此阶段脱氧核糖核酸合成不再发生。在营养缺乏期间，琼脂平板上会启动在液体生长培养基或磷酸镁缓冲液中的不可逆裂解，这种裂解会导致一部分群体诱导形成粘孢子。这种诱导取决于裂解产物的浓度，从而提供了证据表明发育诱导的自溶是粘孢子诱导所必需的。
