Loftus Randy W, Dexter Franklin, Brown Jeremiah R
Anesthesiology and Perioperative Medicine, Mayo Clinic Rochester, Rochester, USA.
Anesthesia, University of Iowa, Iowa City, USA.
Cureus. 2025 Mar 14;17(3):e80590. doi: 10.7759/cureus.80590. eCollection 2025 Mar.
Central laboratory processing of anesthesia work area reservoir samples is used to improve infection control measures. Reservoir samples returning ≥ 100 colony forming units (CFU) and (detection are monitored to identify improvement targets. The impact of sample shipment time under ambient conditions on these meaningful outcomes has not been characterized. Such insight could help to further optimize feedback that has been proven to generate substantial reductions in surgical site infections. In this study, we aimed to assess the impact of ambient shipping conditions on patient intravenous stopcock sample CFU ≥ 100 and detection because stopcock contamination is repeatedly associated with increased patient mortality.
We conducted a retrospective analysis involving seven geographically dispersed hospitals over a 4.2-year (October 1, 2018 to December 31, 2022) study period. We chose geographically dispersed sites considering variation in ambient shipping conditions and time. Stopcocks sampled at the end of surgery were shipped to a central laboratory, plated to sheep's blood agar, incubated for 24hr at 36°C, CFU/mL quantified, and distinct isolates assessed by colony morphology, Gram stain, simple rapid tests (e.g., coagulase, oxidase, lactose fermentation, catalase), and selective growth medium.
A total of 969 stopcock samples were analyzed. The percentage of stopcocks with CFU ≥ 100 was stable following sample collection from days 3 to 32 (odds ratio (OR) 1.0086/day, 95% confidence interval (CI) 0.9868-1.0309/day) and from sample kit preparation from days 3 to 143 (OR 1.0044/day, 95% CI 0.9991-1.0099/day). detection decreased beyond 14 days from the period of collection during the surgical procedure (P = 0.0024; OR 0.83, 95% CI 0.21-0.71).
When utilizing a central laboratory for processing anesthesia workspace reservoir stopcock set samples, there is stability of ≥ 100 CFU for up to 32 days from collection and up to 143 days from kit preparation. detection remains stable for up to 14 days. Therefore, when monitoring stopcock contamination to provide feedback, samples should be processed within 14 days from their collection. Anticipated shipment times should be considered by sample collection personnel to ensure optimal sample yield.
对麻醉工作区储液器样本进行中心实验室处理有助于改进感染控制措施。返回菌落形成单位（CFU）≥100且进行检测的储液器样本会受到监测，以确定改进目标。目前尚未明确环境条件下样本运输时间对这些有意义结果的影响。了解这一点有助于进一步优化反馈，已证实这种反馈能大幅降低手术部位感染率。在本研究中，我们旨在评估环境运输条件对患者静脉输液旋塞样本CFU≥100及检测结果的影响，因为旋塞污染与患者死亡率增加反复相关。
我们进行了一项回顾性分析，研究期为4.2年（2018年10月1日至2022年12月31日），涉及七家地理位置分散的医院。考虑到环境运输条件和时间的差异，我们选择了地理位置分散的地点。手术结束时采集的旋塞样本被运至中心实验室，接种于羊血琼脂平板，在36°C下培养24小时，对CFU/mL进行定量，并通过菌落形态、革兰氏染色、简单快速试验（如凝固酶、氧化酶、乳糖发酵、过氧化氢酶）和选择性生长培养基对不同分离株进行评估。
共分析了969个旋塞样本。从采集后第3天至第32天，CFU≥100的旋塞样本百分比保持稳定（优势比（OR）为1.0086/天，95%置信区间（CI）为0.9868 - 1.0309/天），从样本试剂盒制备后第3天至第143天也保持稳定（OR为1.0044/天，95%CI为0.9991 - 1.0099/天）。在手术过程中采集样本后的14天之后，检测结果下降（P = 0.0024；OR为0.83，95%CI为0.21 - 0.71）。
当利用中心实验室处理麻醉工作区储液器旋塞样本时，从采集起长达32天以及从试剂盒制备起长达143天内，CFU≥100的情况保持稳定。检测结果在长达14天内保持稳定。因此，在监测旋塞污染以提供反馈时，样本应在采集后14天内进行处理。样本采集人员应考虑预期的运输时间，以确保获得最佳样本结果。
