Liu Xiaoxue, Yang Hao, Xie Shanna, Wang Xinyu, Tian Yang, Song Shuang
College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.
Yunnan Key Laboratory of Precision Nutrition and Personalized Food Manufacturing, Yunnan Agricultural University, Kunming, China.
Front Immunol. 2025 Apr 1;16:1546189. doi: 10.3389/fimmu.2025.1546189. eCollection 2025.
Acute lymphoblastic leukemia (T-ALL) is one of the most common hematologic malignancies in children worldwide. Despite advances in chemotherapy in recent years, more than 50% of adult T-ALL patients still experience treatment resistance and relapse/refractory disease. , a new food resource in China, has high nutritional value and various pharmacological activities. protein is one of the main active ingredients in leaves, and some studies have shown that protein has antioxidant, anti-inflammatory, antibacterial, anticancer, and other bioactivities; however, the anti-acute lymphoblastic leukemia (T-ALL) activity of the protein and its mechanism of action are still unclear. In this study, we used leaves protein as the experimental material and used the MTT assay, flow cytometry, and Western blot techniques to study the effects of leaves protein on Jurkat cell growth, apoptosis, and the cell cycle and the underlying molecular mechanism. leaves protein inhibited Jurkat cell proliferation; induced apoptosis and cycle arrest; increased autophagy; and inhibited Jurkat cell proliferation by regulating the MAPK/AKT pathway. The results of this study provide new ideas and a theoretical basis for the development of leaves protein for the prevention and treatment of leukemia.
急性淋巴细胞白血病（T-ALL）是全球儿童中最常见的血液系统恶性肿瘤之一。尽管近年来化疗取得了进展，但仍有超过50%的成年T-ALL患者出现治疗耐药及复发/难治性疾病。[某种食物名称]是中国一种新的食物资源，具有很高的营养价值和多种药理活性。[食物名称]蛋白是[食物名称]叶中的主要活性成分之一，一些研究表明，[食物名称]蛋白具有抗氧化、抗炎、抗菌、抗癌等生物活性；然而，[食物名称]蛋白的抗急性淋巴细胞白血病（T-ALL）活性及其作用机制仍不清楚。在本研究中，我们以[食物名称]叶蛋白为实验材料，采用MTT法、流式细胞术和蛋白质免疫印迹技术，研究[食物名称]叶蛋白对Jurkat细胞生长、凋亡、细胞周期的影响及其潜在分子机制。[食物名称]叶蛋白抑制Jurkat细胞增殖；诱导凋亡和周期阻滞；增加自噬；并通过调节MAPK/AKT途径抑制Jurkat细胞增殖。本研究结果为开发[食物名称]叶蛋白用于白血病的防治提供了新思路和理论依据。
