Carey-Morgan Henry, Polley Nabarun, Korten Till, Pacholski Claudia, Diez Stefan
B CUBE - Center for Molecular Bioengineering, TUD University of Technology Dresden, 01307, Dresden, Germany.
Institute of Chemistry, Physical Chemistry - innoFSPEC, University of Potsdam, Am Mühlenberg 3, 14476, Potsdam, Germany.
Small. 2025 Jun;21(22):e2411836. doi: 10.1002/smll.202411836. Epub 2025 Apr 16.
Prolonged hospital waiting times are linked with increased patient mortality and cause additional financial burdens on institutions. Efficient point-of-care diagnosis would help alleviate this, but is hampered by a lack of cost-effective devices capable of rapid, in situ, wide ranging analyte detection. Lab-on-fiber technology provides an answer allowing for diagnosis, treatment, and monitoring in situ with real time feedback. Here, a device is demonstrated that harnesses motor-protein-driven-microtubule molecular detection assays to optical fibers. By developing a new method for microscope-free microtubule gliding speed determination, proof of concept is demonstrated in the detection of Monomeric Streptavidin and Neutravidin, which initiate a decrease in speed in biotinylated microtubules as well as bundling in the latter case. Utilizing antibody functionalizsed microtubules label-free and microscope-free detection of the heart attack marker Creatine Kinase-MB, as well secondary antibodies in nm concentration is demonstrated. This detector has the potential to be used in situ, providing rapid, low-cost, multiplex analyte screening and detection.
医院候诊时间过长与患者死亡率增加相关，并给医疗机构带来额外的经济负担。高效的即时诊断将有助于缓解这一问题，但由于缺乏能够进行快速、原位、广泛分析物检测的经济高效设备而受到阻碍。光纤实验室技术提供了一种解决方案，能够实现原位诊断、治疗和监测，并提供实时反馈。在此，展示了一种将基于运动蛋白驱动微管的分子检测方法应用于光纤的设备。通过开发一种无需显微镜即可测定微管滑动速度的新方法，在检测单体链霉亲和素和中性抗生物素蛋白时证明了概念验证，这两种蛋白会使生物素化微管的速度降低，在后一种情况下还会导致微管成束。利用抗体功能化微管实现了对心脏病发作标志物肌酸激酶同工酶MB的无标记、无需显微镜检测，以及对纳摩尔浓度二抗的检测。这种探测器有潜力用于原位检测，提供快速、低成本的多分析物筛选和检测。
