Wang Shaoshuai, Wang Junmin, Zhang Xinru, Xu Shijun, Peng Qinfeng, Li Yifei, Ding Ruoqi, Jiang Bing, Wang Shuyu, Zhang Shuaibing, Hu Siyuan, Rastegar-Kashkooli Yousef, Xing Na, Li Nan, Wang Menglu, Wang Junyang, Chen Xuemei, Jiang Chao, Fan Xiaochong, Wang Jian
Department of Pain Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Department of Human Anatomy, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.
CNS Neurosci Ther. 2025 Apr;31(4):e70372. doi: 10.1111/cns.70372.
The utilization of anti-inflammatory therapy for treating brain diseases holds promise; however, research on intranasal administration of drug compounds remains limited. Quantitative data, particularly pharmacokinetics, are scant, and direct evidence of the distribution of intranasally administered recombinant interleukin 10 (rIL-10) within the brain is lacking.
Employing fluorescent labeling, in vivo imaging, and confocal microscopy, we meticulously monitored the distribution and delivery pathways of intranasally administered rIL-10 in the brain.
Our findings demonstrate that rIL-10 can permeate the blood-brain barrier and reach the perihematomal area in the striatum of mice with intracerebral hemorrhage. Intranasally administered rIL-10 primarily targets the cerebral cortex, striatum, and thalamus, traversing the olfactory nerve pathway and perivascular space to access these brain regions. This mode of delivery effectively mitigated secondary brain injury after intracerebral hemorrhage. This study contributes to intranasal drug delivery research, offering compelling evidence to support the intranasal delivery of anti-inflammatory cytokines or alternative drug candidates for treating brain diseases.
利用抗炎疗法治疗脑部疾病具有前景；然而，关于药物化合物鼻内给药的研究仍然有限。定量数据，尤其是药代动力学数据匮乏，且缺乏鼻内给药的重组白细胞介素10（rIL-10）在脑内分布的直接证据。
采用荧光标记、体内成像和共聚焦显微镜技术，我们精心监测了鼻内给药的rIL-10在脑内的分布和递送途径。
我们的研究结果表明，rIL-10能够穿透血脑屏障并到达脑出血小鼠纹状体的血肿周围区域。鼻内给药的rIL-10主要靶向大脑皮层、纹状体和丘脑，通过嗅觉神经途径和血管周围间隙进入这些脑区。这种递送方式有效减轻了脑出血后的继发性脑损伤。本研究为鼻内给药研究做出了贡献，提供了有力证据支持鼻内递送抗炎细胞因子或其他治疗脑部疾病的候选药物。
