As the predominant constituents of the vaginal microbiome in healthy women, species are considered essential in maintaining a homeostatic vaginal microbiome. Specific species can produce beneficial metabolites to support their persistence within the host environment and inhibit colonization. Due to the extensive diversity of species and their metabolites, comprehensively investigating all possible interactions remains challenging. This study employed an integrative approach combining genome-scale metabolic modeling, metagenomic sequencing, and validation to explore and interactions. Pairwise simulations of 159 strains with revealed that most strains exhibit inhibitory effects, altering fungal amino acid and carbohydrate metabolism. Key inhibitory metabolites identified included formate, L-lactate, and L-malate. Metagenomic analysis of vaginal swabs from 20 vulvovaginal candidiasis (VVC) patients and 20 healthy women showed a correlation between species abundance and reduced colonization. experiments confirmed the inhibitory effects of these metabolites and the selected strains on growth, thereby validating our computational predictions. These findings provide insights into the metabolic interactions within the vaginal microbiome and pave the way for targeted microbial or metabolite-based therapeutic strategies to manage VVC.IMPORTANCEVulvovaginal candidiasis is a prevalent fungal infection with significant implications for women's health, caused primarily by . Although the protective role of a -dominated vaginal microbiome is well established, the metabolic mechanisms underlying the interactions between species and remain inadequately understood. Specifically, the species that effectively inhibit and the metabolic pathways involved warrant further investigation. This study offers novel insights into the metabolic mechanisms underlying antagonism against . By identifying critical metabolic pathways and inhibitory metabolites, this study enhances our understanding of vaginal microbiome dynamics and host-microbe interactions. The findings suggest that key strains and their metabolites could significantly reduce harmful levels of , paving the way for future therapeutic strategies that leverage these microbial characteristics to promote vaginal health.