Cheong W C, Yap H H
Southeast Asian J Trop Med Public Health. 1985 Mar;16(1):54-8.
The pathogenicity of Bacillus sphaericus strain 1593 was tested against laboratory-reared larvae of four local species of mosquitoes of public health importance in Malaysia; Aedes aegypti, Anopheles balabacensis, Mansonia uniformis and Culex quinquefasciatus. The bacteria was shake-cultured at 28 +/- 1 degrees C for three days, using Glucose-Yeast Extract Salts medium. After which, the spores and vegetative cells were harvested and stored at 4 degrees C before use. Conditions for bioassays were mean temperature of 25 +/- 1 degrees C and relative humidity 65 +/- 5.0. Twenty third-instar larvae of each species were assayed in 90 ml of diluted spore solution. Each concentration and a control were replicated three times for each bioassay. Larval mortalities at 24 hours and 48 hours were taken and analyzed through Probit Analysis using a computer (IBM 370). LC50 values after 48 hours of exposure showed an increasing order of larval susceptibility as follows: Ae. aegypti (417.70 x 10(4)), An. balabacensis (45.84 x 10(4)), Ma. uniformis (18.23 x 10(4)) and Cx. quinquefasciatus (4.14 x 10(4) spores/ml). With the ability to kill 90% of the Cx. quinquefasciatus larvae tested with just a concentration of 10(5) spores/ml, B. sphaericus (strain 1593) has shown good potential as a biocontrol agent for this species of mosquito.
对球形芽孢杆菌1593菌株针对马来西亚四种具有公共卫生重要性的本地蚊虫实验室饲养幼虫的致病性进行了测试；埃及伊蚊、巴拉巴按蚊、单色曼蚊和致倦库蚊。使用葡萄糖 - 酵母提取物盐培养基，将细菌在28±1℃振荡培养三天。之后，收获孢子和营养细胞并在4℃下储存备用。生物测定条件为平均温度25±1℃和相对湿度65±5.0。每种蚊虫的20只三龄幼虫在90毫升稀释的孢子溶液中进行测定。每次生物测定中，每个浓度和一个对照重复三次。记录24小时和48小时的幼虫死亡率，并使用计算机（IBM 370）通过概率分析进行分析。暴露48小时后的LC50值显示幼虫易感性的增加顺序如下：埃及伊蚊（417.70×10⁴）、巴拉巴按蚊（45.84×10⁴）、单色曼蚊（18.23×10⁴）和致倦库蚊（4.14×10⁴个孢子/毫升）。球形芽孢杆菌（1593菌株）仅用10⁵个孢子/毫升的浓度就能杀死90%的受试致倦库蚊幼虫，显示出作为这种蚊虫生物防治剂的良好潜力。
