Liu Kanzhen, Pang Cuiping, Li Qinghua, Li Jianghua, Du Guocheng, Zhang Guoqiang
Science Center for Future Foods, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
Foods. 2025 Mar 29;14(7):1209. doi: 10.3390/foods14071209.
Bioactive components of abalone and other marine organisms have attracted significant attention owing to their functional performance. The development of peptides with bioactivity like angiotensin-converting enzyme inhibitory (ACEi) and antioxidant properties is of great significance for chronic disease management and drug discovery. In this study, according to the issues of low utilization rate and bioactive content from the hydrolysate of abalone, single-factor and orthogonal experiments were designed to improve the utilization rate of abalone protein, and step hydrolysis with specific proteases was used to improve the overall biological activity of the hydrolysate. A total of 1937 peptide sequences were obtained from the highly bioactive components after separation and peptidomic analysis. Through virtual screening and molecular docking, 14 peptides exhibiting ACEi activity were identified and synthesized for experimental verification, with IC values ranging from 0.05 to 0.54 mg/mL. Notably, nine of these peptides were powerful antioxidants. The developed step enzymatic hydrolysis and screening-assisted preparation also provided a feasible and efficient method for exploring more bioactive peptides from diverse biomasses.
鲍鱼及其他海洋生物的生物活性成分因其功能特性而备受关注。开发具有血管紧张素转换酶抑制（ACEi）和抗氧化等生物活性的肽，对于慢性病管理和药物发现具有重要意义。本研究针对鲍鱼水解产物利用率低和生物活性成分含量低的问题，设计单因素和正交实验以提高鲍鱼蛋白利用率，并采用特定蛋白酶分步水解来提高水解产物的整体生物活性。经分离和肽组学分析，从高生物活性成分中总共获得了1937个肽序列。通过虚拟筛选和分子对接，鉴定并合成了14个具有ACEi活性的肽进行实验验证，其IC值范围为0.05至0.54mg/mL。值得注意的是，其中9个肽是强大的抗氧化剂。所开发的分步酶解和筛选辅助制备方法，也为从多种生物质中探索更多生物活性肽提供了一种可行且高效的方法。
